| Literature DB >> 16233276 |
Takefumi Sone1, Eiji Nagamori, Tomohiko Ikeuchi, Atsushi Mizukami, Yukiko Takakura, Shin'ichiro Kajiyama, Ei-Ichiro Fukusaki, Satoshi Harashima, Akio Kobayashi, Kiichi Fukui.
Abstract
We have produced micrometer-sized calcium alginate beads referred to as "bio-beads" that encapsulate plasmid DNA molecules carrying a reporter gene. In order to evaluate the efficiency of the bio-beads in mediating genetic transfection, protoplasts isolated from cultured tobacco cells (BY-2) were transfected with bio-beads containing a plasmid that carries the modified green fluorescent protein gene CaMV35S-sGFP. With the bio-beads treatment, approximately ten-fold higher GFP expression was observed after 24 h incubation compared to that with the conventional method using a naked plasmid solution. Transfection was up to 0.22% efficient. These results indicate that bio-beads have a possibility for efficient transformation in plants.Entities:
Year: 2002 PMID： 16233276 DOI： 10.1263/jbb.94.87
Source DB: PubMed Journal: J Biosci Bioeng ISSN： 1347-4421 Impact factor: 2.894