Literature DB >> 16233101

Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-test) and improvement of mutagen-sensitivity.

R Arai1, Y Makita, Y Oda, T Nagamune.   

Abstract

Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu promoter, thereby improving the sensitivity of the umu-test.

Entities:  

Year:  2001        PMID： 16233101     DOI： 10.1263/jbb.92.301

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  4 in total

1.  New method for evaluation of genotoxicity, based on the use of real-time PCR and lysogenic gram-positive and gram-negative bacteria.

Authors:  Nora Soberón; Rebeca Martín; Juan E Suárez
Journal:  Appl Environ Microbiol       Date:  2007-03-02       Impact factor: 4.792

2.  UDP-glucosyltransferase71c5, a major glucosyltransferase, mediates abscisic acid homeostasis in Arabidopsis.

Authors:  Zhen Liu; Jin-Ping Yan; De-Kuan Li; Qin Luo; Qiujie Yan; Zhi-Bin Liu; Li-Ming Ye; Jian-Mei Wang; Xu-Feng Li; Yi Yang
Journal:  Plant Physiol       Date:  2015-02-20       Impact factor: 8.340

Review 3.  Bacterial genotoxicity bioreporters.

Authors:  Alva Biran; Sharon Yagur-Kroll; Rami Pedahzur; Sebastian Buchinger; Georg Reifferscheid; Hadar Ben-Yoav; Yosi Shacham-Diamand; Shimshon Belkin
Journal:  Microb Biotechnol       Date:  2009-12-29       Impact factor: 5.813

4.  Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation.

Authors:  Sharon Yagur-Kroll; Benny Bilic; Shimshon Belkin
Journal:  Microb Biotechnol       Date:  2009-08-26       Impact factor: 5.813
  4 in total

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