| Literature DB >> 16231599 |
Xiaohong Wang1, Yongnian Yan, Feng Lin, Zhuo Xiong, Rendong Wu, Renji Zhang, Qingping Lu.
Abstract
A new type of collagen/chitosan/heparin matrix, fabricated by gelation of collagen/ chitosan with heparin sodium containing ammonia, was produced to construct livers by tissue engineering and regenerative engineering. The obtained collagen/chitosan/heparin matrix was found to be highly porous, swelled rapidly in PBS solution and was stable in vitro for at least 60 days in collagenase/lysozyme containing buffered aqueous solution (PBS, pH 7.4) at 37 degrees C. The collagen/chitosan/heparin matrix resulted in a superior blood compatibility compared to the ammonia-treated collagen and collagen/chitosan matrices. The morphology and behavior of the cells on the collagen/chitosan/heparin membrane were found to be similar to those on the collagen membrane but different from those on the collagen/chitosan membrane. Hepatocytes cultured on the collagen/chitosan/heparin matrices exhibited highest urea and triglyceride secretion functions 25 days post seeding. These results suggest that this collagen/chitosan/heparin matrix is a potential candidate for liver tissue engineering.Entities:
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Year: 2005 PMID： 16231599 DOI： 10.1163/1568562054798554
Source DB: PubMed Journal: J Biomater Sci Polym Ed ISSN： 0920-5063 Impact factor: 3.517