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Literature DB >> 16228508

Rapid one-step purification of the BChl-a containing FMO-protein from the green sulfur bacterium Chlorobium tepidum using a high efficiency immunomatrix.

Abstract

A new and rapid procedure has been developed for the isolation of the bacteriochlorophyll a-containing Fenna-Matthews-Olson (FMO)-protein from green sulfur bacteria. Polyclonal antibodies raised against the FMO-protein of Chlorobium (Chl.) tepidum were employed in the preparation of an antibody column utilizing immobilized protein A as the matrix. The antibody column afforded essentially a one-step purification process, resulting in preparations that were free from contaminating pigments and proteins. This was evidenced by absorption spectroscopy, SDS-PAGE, and fluorescence emission.

Entities: Chemical Species

Year: 2002 PMID： 16228508 DOI： 10.1023/A:1014907731595

Source DB: PubMed Journal: Photosynth Res ISSN： 0166-8595 Impact factor: 3.573

14 in total

1. Low-temperature spectroscopy of isolated FMO-protein and a membrane-free reaction center complex from the green sulfur bacteriumChlorobium tepidum.

Authors: M Miller; R P Cox; J M Olson
Journal: Photosynth Res Date: 1994-07 Impact factor: 3.573

2. Ultrafast absorption difference spectra of the Fenna-Matthews-Olson protein at 19 K: experiment and simulations.

Authors: D R Buck; S Savikhin; W S Struve
Journal: Biophys J Date: 1997-01 Impact factor: 4.033

3. An improved method for immobilizing IgG antibodies on protein A-agarose.

Authors: T H Sisson; C W Castor
Journal: J Immunol Methods Date: 1990-03-09 Impact factor: 2.303

4. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors: H Schägger; G von Jagow
Journal: Anal Biochem Date: 1987-11-01 Impact factor: 3.365

5. Effects of denaturants on the absorption spectrum of the bacteriochlorophyll-protein from the photosynthetic bacterium Chlorospeudomonas ethylicum.

Authors: A K Ghosh; J M Olson
Journal: Biochim Biophys Acta Date: 1968-07-16

Rapid one-step purification of the BChl-a containing FMO-protein from the green sulfur bacterium Chlorobium tepidum using a high efficiency immunomatrix.

1. Low-temperature spectroscopy of isolated FMO-protein and a membrane-free reaction center complex from the green sulfur bacteriumChlorobium tepidum.

2. Ultrafast absorption difference spectra of the Fenna-Matthews-Olson protein at 19 K: experiment and simulations.

3. An improved method for immobilizing IgG antibodies on protein A-agarose.

4. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

5. Effects of denaturants on the absorption spectrum of the bacteriochlorophyll-protein from the photosynthetic bacterium Chlorospeudomonas ethylicum.

6. The complete amino acid sequence of a bacteriochlorophyll a-protein from Prosthecochloris aestuarii.

Review 7. Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera.

8. A simplification of the protein assay method of Lowry et al. which is more generally applicable.

9. Redox effects on the bacteriochlorophyll a-containing Fenna-Matthews-Olson protein from Chlorobium tepidum.

10. Crystal structure of the bacteriochlorophyll a protein from Chlorobium tepidum.

1. The structure of the FMO protein from Chlorobium tepidum at 2.2 A resolution.