BACKGROUND/AIMS: IFNalpha is an approved treatment option for patients chronically infected with the hepatitis B and C viruses. Additionally, there is an indication for tumor therapy. The exact mechanisms underlying the antiviral and antitumor effects of IFNalpha are not completely understood. In this study, we investigated if the pro-apoptotic factor caspase-8 is a target gene of IFNalpha signalling. METHODS: Huh7 hepatoma cells were used for measuring caspase-8 promoter activity in luciferase reporter assays after IFNalpha stimulation. Caspase-8 expression was monitored by RT-PCR, immunoblotting and measurement of enzymatic activity. Functional caspase-8 promoter elements were identified in gelshift assays and by site directed mutagenesis. Caspase-8 was inhibited using siRNA. RESULTS: IFNalpha treatment induced caspase-8 promoter activity and mRNA expression. We identified a unique promoter element mediating the IFNalpha-dependent increase in caspase-8 transcription. Up-regulation of caspase-8 expression by IFNalpha had no impact on the rate of apoptosis per se. However, co-stimulation with IFNalpha doubled TRAIL-mediated apoptosis and enzymatic caspase-8 activity. The synergistic effect of TRAIL and IFNalpha could be blocked by inhibiting caspase-8 expression. CONCLUSIONS: We demonstrate that caspase-8 is a target gene of IFNalpha and provide evidence showing that IFNalpha treatment sensitizes cells for apoptosis via enhanced caspase-8 transcription.
BACKGROUND/AIMS: IFNalpha is an approved treatment option for patients chronically infected with the hepatitis B and C viruses. Additionally, there is an indication for tumor therapy. The exact mechanisms underlying the antiviral and antitumor effects of IFNalpha are not completely understood. In this study, we investigated if the pro-apoptotic factor caspase-8 is a target gene of IFNalpha signalling. METHODS: Huh7 hepatoma cells were used for measuring caspase-8 promoter activity in luciferase reporter assays after IFNalpha stimulation. Caspase-8 expression was monitored by RT-PCR, immunoblotting and measurement of enzymatic activity. Functional caspase-8 promoter elements were identified in gelshift assays and by site directed mutagenesis. Caspase-8 was inhibited using siRNA. RESULTS:IFNalpha treatment induced caspase-8 promoter activity and mRNA expression. We identified a unique promoter element mediating the IFNalpha-dependent increase in caspase-8 transcription. Up-regulation of caspase-8 expression by IFNalpha had no impact on the rate of apoptosis per se. However, co-stimulation with IFNalpha doubled TRAIL-mediated apoptosis and enzymatic caspase-8 activity. The synergistic effect of TRAIL and IFNalpha could be blocked by inhibiting caspase-8 expression. CONCLUSIONS: We demonstrate that caspase-8 is a target gene of IFNalpha and provide evidence showing that IFNalpha treatment sensitizes cells for apoptosis via enhanced caspase-8 transcription.
Authors: S L Petersen; T T Chen; D A Lawrence; S A Marsters; F Gonzalvez; A Ashkenazi Journal: Cell Death Differ Date: 2015-04-17 Impact factor: 15.828
Authors: Michael A Joyce; Kathie-Anne Walters; Sue-Ellen Lamb; Mathew M Yeh; Lin-Fu Zhu; Norman Kneteman; Jason S Doyle; Michael G Katze; D Lorne Tyrrell Journal: PLoS Pathog Date: 2009-02-06 Impact factor: 6.823