| Literature DB >> 1621990 |
M E Adams1, D Q Huang, L Y Yao, L J Sandell.
Abstract
We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 M guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification.Entities:
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Year: 1992 PMID： 1621990 DOI： 10.1016/0003-2697(92)90211-o
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365