Literature DB >> 1621959

A colorimetric method for the quantitation of uronic acids and a specific assay for galacturonic acid.

K A Taylor1, J G Buchanan-Smith.   

Abstract

A method of quantitating uronic acids and uronic acids from pectin in particular is described. The method uses carbazole in 80% sulfuric acid with borate ions added. The assay is carried out at 60 degrees C. This assay has some cross reactivity with aldose sugars and must be timed precisely. A further method that is specific for galacturonic acid is also described. This method uses concentrated sulfuric acid and carbazole only. Of the biological substances tested, only formaldehyde and glyceraldehyde showed a reactivity of more than 10% that of galacturonic acid on a weight to weight basis.

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Year:  1992        PMID： 1621959     DOI： 10.1016/0003-2697(92)90194-c

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

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