PURPOSE: We investigated the effects of recombinant human (rh) erythropoietin (EPO) on erectile function recovery in a rat model of cavernous nerve (CN) injury. MATERIALS AND METHODS: Male rats underwent unilateral CN transection and excision of a 5 mm segment of the contralateral CN. One group received rhEPO (5,000 U/kg) subcutaneously daily for 14 days, while another received rhEPO 1 day and 1 hour prior to nerve injury. An additional group of animals was pretreated with 1 dose of darbepoetin (25 microg/kg). At 14 days following CN injury rats underwent erection physiology studies. Axonal regeneration was evaluated by electron microscopy. EPO receptor expression in the penis and major pelvic ganglion was evaluated immunohistochemically and by real-time polymerase chain reaction. RESULTS: Daily rhEPO effectively recovered erections after CN injury compared with saline treatment. Maximal intracavernous pressure area under the curve normalized to mean arterial pressure was significantly greater in EPO treated vs saline treated animals (p < 0.05). rhEPO and darbepoetin pretreatment was as effective as continuous 14-day therapy. EPO receptor expression was localized to neuronal cell bodies of the major pelvic ganglion, penile nerves and endothelial cells in the penis. Electron microscopy revealed significant improvement in axonal regeneration in rhEPO treated animals 14 days following injury compared to controls. CONCLUSIONS: EPO receptors are expressed in local neuronal and vascular tissues. Exogenous administration of rhEPO or darbepoetin in the setting of CN injury promotes erectile function recovery. This occurs through axonal regeneration of the injured nerve and possible penile protection.
PURPOSE: We investigated the effects of recombinant human (rh) erythropoietin (EPO) on erectile function recovery in a rat model of cavernous nerve (CN) injury. MATERIALS AND METHODS: Male rats underwent unilateral CN transection and excision of a 5 mm segment of the contralateral CN. One group received rhEPO (5,000 U/kg) subcutaneously daily for 14 days, while another received rhEPO 1 day and 1 hour prior to nerve injury. An additional group of animals was pretreated with 1 dose of darbepoetin (25 microg/kg). At 14 days following CN injuryrats underwent erection physiology studies. Axonal regeneration was evaluated by electron microscopy. EPO receptor expression in the penis and major pelvic ganglion was evaluated immunohistochemically and by real-time polymerase chain reaction. RESULTS: Daily rhEPO effectively recovered erections after CN injury compared with saline treatment. Maximal intracavernous pressure area under the curve normalized to mean arterial pressure was significantly greater in EPO treated vs saline treated animals (p < 0.05). rhEPO and darbepoetin pretreatment was as effective as continuous 14-day therapy. EPO receptor expression was localized to neuronal cell bodies of the major pelvic ganglion, penile nerves and endothelial cells in the penis. Electron microscopy revealed significant improvement in axonal regeneration in rhEPO treated animals 14 days following injury compared to controls. CONCLUSIONS:EPO receptors are expressed in local neuronal and vascular tissues. Exogenous administration of rhEPO or darbepoetin in the setting of CN injury promotes erectile function recovery. This occurs through axonal regeneration of the injured nerve and possible penile protection.
Authors: Kalpesh T Vakharia; Robin W Lindsay; Christopher Knox; Colin Edwards; Doug Henstrom; Julie Weinberg; Tessa A Hadlock; James T Heaton Journal: Otolaryngol Head Neck Surg Date: 2011-01 Impact factor: 3.497
Authors: Nicholas L Angeloni; Christopher W Bond; Yi Tang; Daniel A Harrington; Shuming Zhang; Samuel I Stupp; Kevin E McKenna; Carol A Podlasek Journal: Biomaterials Date: 2010-10-23 Impact factor: 12.479
Authors: J A Lohmeyer; E Essmann; S J Richerson; C Hagel; J T Egana; A Condurache; P Ganske; K Schulz; P Mailänder; H G Machens Journal: Langenbecks Arch Surg Date: 2008-02-26 Impact factor: 3.445
Authors: Arthur L Burnett; Sena F Sezen; Ahmet Hoke; Anthony O Caggiano; Jennifer Iaci; Gwen Lagoda; Biljana Musicki; Anthony J Bella Journal: J Sex Med Date: 2015-01-30 Impact factor: 3.802
Authors: R Kato; D Wolfe; C H Coyle; J B Wechuck; P Tyagi; T Tsukamoto; J B Nelson; J C Glorioso; M B Chancellor; N Yoshimura Journal: Gene Ther Date: 2008-07-31 Impact factor: 5.250