| Literature DB >> 16214271 |
Patrick J Blair1, Tadeusz J Kochel, Kanakatte Raviprakash, Carolina Guevara, Milagros Salazar, Shuenn-Jue Wu, James G Olson, Kevin R Porter.
Abstract
A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.Entities:
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Year: 2005 PMID： 16214271 DOI： 10.1016/j.vaccine.2005.09.032
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641