Varsha Gupta1, Priya Datta, Jagdish Chander. 1. Department of Microbiology, Government Medical College and Hospital, 1154 A, Sec-32 B, Chandigarh 160030, India. varshagupta_99@yahoo.com
Abstract
OBJECTIVE: Gram-negative bacteria with acquired metallo-beta lactamases production have been increasingly reported in some countries, necessitating their detection. We determined the occurrence of acquired metallo-beta lactamases (MBL) producing bacteria in our teaching hospital. METHOD: The method employed was a rapid technique using a disk of imipenem plus 750 microg of EDTA, on Mueller-Hinton plate which was supplemented with 70 microg/ml of zinc sulfate, to differentiate MBL producing Pseudomonads and Acinetobacter. RESULT: Out of 200 isolates tested, 7.5% of our Pseudomonads and Acinetobacter were shown to be MBL producers. CONCLUSIONS: Though MBL prevalence is still low in our hospital set up, screening for MBL among Pseudomonas and Acinetobacter isolates should be routinely done in laboratory.
OBJECTIVE: Gram-negative bacteria with acquired metallo-beta lactamases production have been increasingly reported in some countries, necessitating their detection. We determined the occurrence of acquired metallo-beta lactamases (MBL) producing bacteria in our teaching hospital. METHOD: The method employed was a rapid technique using a disk of imipenem plus 750 microg of EDTA, on Mueller-Hinton plate which was supplemented with 70 microg/ml of zinc sulfate, to differentiate MBL producing Pseudomonads and Acinetobacter. RESULT: Out of 200 isolates tested, 7.5% of our Pseudomonads and Acinetobacter were shown to be MBL producers. CONCLUSIONS: Though MBL prevalence is still low in our hospital set up, screening for MBL among Pseudomonas and Acinetobacter isolates should be routinely done in laboratory.
Authors: Darren Wong; Travis B Nielsen; Robert A Bonomo; Paul Pantapalangkoor; Brian Luna; Brad Spellberg Journal: Clin Microbiol Rev Date: 2017-01 Impact factor: 26.132