| Literature DB >> 16211501 |
Sung-Hou Kim1, Dong Hae Shin, Jinyu Liu, Vaheh Oganesyan, Shengfeng Chen, Qian Steven Xu, Jeong-Sun Kim, Debanu Das, Ursula Schulze-Gahmen, Stephen R Holbrook, Elizabeth L Holbrook, Bruno A Martinez, Natalia Oganesyan, Andy DeGiovanni, Yun Lou, Marlene Henriquez, Candice Huang, Jaru Jancarik, Ramona Pufan, In-Geol Choi, John-Marc Chandonia, Jingtong Hou, Barbara Gold, Hisao Yokota, Steven E Brenner, Paul D Adams, Rosalind Kim.
Abstract
The initial aim of the Berkeley Structural Genomics Center is to obtain a near-complete structural complement of two minimal organisms, closely related pathogens Mycoplasma genitalium and M. pneumoniae. The former has fewer than 500 genes and the latter fewer than 700 genes. To achieve this goal, the current protein targets have been selected starting with those predicted to be most tractable and likely to yield new structural and functional information. During the past 3 years, the semi-automated structural genomics pipeline has been set up from cloning, expression, purification, and ultimately to structural determination. The results from the pipeline substantially increased the coverage of the protein fold space of M. pneumoniae and M. genitalium. Furthermore, about 1/2 of the structures of 'unique' protein sequences revealed new and novel folds, and over 2/3 of the structures of previously annotated 'hypothetical proteins' inferred their molecular functions.Entities:
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Year: 2005 PMID： 16211501 DOI： 10.1007/s10969-005-2651-9
Source DB: PubMed Journal: J Struct Funct Genomics ISSN： 1345-711X