OBJECTIVE: To study the expression of plexin-B1 in the mouse ovary and its possible role in follicular development. DESIGN: Controlled laboratory study. SETTING: Research laboratory, Ha'Emek Medical Center. PATIENT(S): ICR female mice (n = 36) were studied. MAIN OUTCOME MEASURE(S): Plexin-B1 expression by immunohistochemistry, Western blot analysis, and reverse transcriptase-polymerase chain reaction. Microscopic morphometric and morphologic analysis for the evaluation of mouse follicular growth and E2, P, and T secretion from the developed follicles. RESULT(S): Plexin-B1 was expressed in the mouse ovary mostly in the granulosa cells. Plexin-B1 gene expression increased significantly by 126.8% +/- 38% in the pregnant mare serum gonadotropin-treated group and increased by 98.7% +/- 15.2 and 262.2% +/- 65.4% in the hCG-treated group 6 h and 24 h after injection, respectively (ANOVA; P < .05). Comparison of the maximum diameters of the control and study groups on day 9 of the culture period revealed a significant difference between the two groups (P < .001). In the control group, the mean maximum diameter was 191 +/- 2.6 microm compared with 136 +/- 5.13 microm in the presence of neutralizing antibodies against plexin-B1. Neutralizing antibody against plexin-B1 decreases E2 and P secretion. CONCLUSION(S): This study shows for the first time the expression of plexin-B1 in the mouse ovary. A possible role for plexin-B1 in follicular growth and steroidogenesis, most likely via the granulosa cells, is suggested.
OBJECTIVE: To study the expression of plexin-B1 in the mouse ovary and its possible role in follicular development. DESIGN: Controlled laboratory study. SETTING: Research laboratory, Ha'Emek Medical Center. PATIENT(S): ICR female mice (n = 36) were studied. MAIN OUTCOME MEASURE(S): Plexin-B1 expression by immunohistochemistry, Western blot analysis, and reverse transcriptase-polymerase chain reaction. Microscopic morphometric and morphologic analysis for the evaluation of mouse follicular growth and E2, P, and T secretion from the developed follicles. RESULT(S): Plexin-B1 was expressed in the mouse ovary mostly in the granulosa cells. Plexin-B1 gene expression increased significantly by 126.8% +/- 38% in the pregnant mare serum gonadotropin-treated group and increased by 98.7% +/- 15.2 and 262.2% +/- 65.4% in the hCG-treated group 6 h and 24 h after injection, respectively (ANOVA; P < .05). Comparison of the maximum diameters of the control and study groups on day 9 of the culture period revealed a significant difference between the two groups (P < .001). In the control group, the mean maximum diameter was 191 +/- 2.6 microm compared with 136 +/- 5.13 microm in the presence of neutralizing antibodies against plexin-B1. Neutralizing antibody against plexin-B1 decreases E2 and P secretion. CONCLUSION(S): This study shows for the first time the expression of plexin-B1 in the mouse ovary. A possible role for plexin-B1 in follicular growth and steroidogenesis, most likely via the granulosa cells, is suggested.
Authors: Kelly E Roney; Brian P O'Connor; Haitao Wen; Eda K Holl; Elizabeth H Guthrie; Beckley K Davis; Stephen W Jones; Sushmita Jha; Lisa Sharek; Rafael Garcia-Mata; James E Bear; Jenny P-Y Ting Journal: PLoS One Date: 2011-09-23 Impact factor: 3.240