OBJECTIVE: To establish a novel endometriosis model that allows for repetitive in vivo analyses of angiogenesis in ectopic endometrial tissue. DESIGN: Intravital fluorescence microscopic study. SETTING: Institute for Clinical and Experimental Surgery, University of Saarland. ANIMAL(S): Female Syrian golden hamsters equipped with skinfold chambers. INTERVENTION(S): Large (0.5 mm2) and small (0.1 mm2) endometrial fragments were mechanically isolated and transplanted autologously into skinfold chambers of untreated hormonally synchronized or bilaterally ovariectomized hamsters. MAIN OUTCOME MEASURE(S): Angiogenesis, vascularization, and microhemodynamics were analyzed over a 14-day period. RESULT(S): In untreated controls, endometrial fragments developed complete microvascular networks during the experimental observation period. Interestingly, microvascular blood flow was higher in large than in small fragments. Histologic examinations revealed proliferating endometriosis-like lesions with dilated endometrial glands surrounded by a richly vascularized stroma. Vascularization of endometrial fragments in synchronized animals did not differ from that of untreated controls. In contrast, endometrial fragments in ovariectomized animals showed a delay in angiogenesis and a significantly decreased blood perfusion, indicating the essential role of ovarian estrogens for ectopic vascularization and perfusion of endometrial tissue. CONCLUSION(S): This novel model of endometrial tissue transplantation is a useful experimental approach, not only to focus on the in vivo pathogenesis of endometriosis but also to develop antiangiogenic strategies for the treatment of this disease.
OBJECTIVE: To establish a novel endometriosis model that allows for repetitive in vivo analyses of angiogenesis in ectopic endometrial tissue. DESIGN: Intravital fluorescence microscopic study. SETTING: Institute for Clinical and Experimental Surgery, University of Saarland. ANIMAL(S): Female Syrian golden hamsters equipped with skinfold chambers. INTERVENTION(S): Large (0.5 mm2) and small (0.1 mm2) endometrial fragments were mechanically isolated and transplanted autologously into skinfold chambers of untreated hormonally synchronized or bilaterally ovariectomized hamsters. MAIN OUTCOME MEASURE(S): Angiogenesis, vascularization, and microhemodynamics were analyzed over a 14-day period. RESULT(S): In untreated controls, endometrial fragments developed complete microvascular networks during the experimental observation period. Interestingly, microvascular blood flow was higher in large than in small fragments. Histologic examinations revealed proliferating endometriosis-like lesions with dilated endometrial glands surrounded by a richly vascularized stroma. Vascularization of endometrial fragments in synchronized animals did not differ from that of untreated controls. In contrast, endometrial fragments in ovariectomized animals showed a delay in angiogenesis and a significantly decreased blood perfusion, indicating the essential role of ovarian estrogens for ectopic vascularization and perfusion of endometrial tissue. CONCLUSION(S): This novel model of endometrial tissue transplantation is a useful experimental approach, not only to focus on the in vivo pathogenesis of endometriosis but also to develop antiangiogenic strategies for the treatment of this disease.
Authors: Matthias W Laschke; Christian Giebels; Ruth M Nickels; Claudia Scheuer; Michael D Menger Journal: Am J Pathol Date: 2010-12-23 Impact factor: 4.307
Authors: Jeannette Rudzitis-Auth; Sophia A Fuß; Vivien Becker; Michael D Menger; Matthias W Laschke Journal: Br J Pharmacol Date: 2020-04-12 Impact factor: 8.739
Authors: Carla N Olivares; Laura D Alaniz; Michael D Menger; Rosa I Barañao; Matthias W Laschke; Gabriela F Meresman Journal: PLoS One Date: 2016-03-28 Impact factor: 3.240