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Literature DB >> 16208019

Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections.

Maricel Vidal¹, Eileen Kruger, Claudia Durán, Rosanna Lagos, Myron Levine, Valeria Prado, Cecilia Toro, Roberto Vidal.

Abstract

We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.

Entities: Disease Species

Mesh：

Substances：

Year: 2005 PMID： 16208019 PMCID： PMC1248459 DOI： 10.1128/JCM.43.10.5362-5365.2005

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

17 in total

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Authors: Roberto Vidal; Maricel Vidal; Rossana Lagos; Myron Levine; Valeria Prado
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Journal: Infect Immun Date: 1998-10 Impact factor: 3.441

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3. Infectious diarrhoea in antiretroviral therapy-naive HIV/AIDS patients in Kenya.

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4. Shiga toxin-producing Escherichia coli O100:H⁻: stx2e in drinking water contaminated by waste water in Finland.

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5. Escherichia coli isolates from inflammatory bowel diseases patients survive in macrophages and activate NLRP3 inflammasome.

Authors: Marjorie De la Fuente; Luigi Franchi; Daniela Araya; David Díaz-Jiménez; Mauricio Olivares; Manuel Álvarez-Lobos; Douglas Golenbock; María-Julieta González; Francisco López-Kostner; Rodrigo Quera; Gabriel Núñez; Roberto Vidal; Marcela A Hermoso
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10. Detection of Escherichia coli, Salmonella spp., Shigella spp., Yersinia enterocolitica, Vibrio cholerae, and Campylobacter spp. enteropathogens by 3-reaction multiplex polymerase chain reaction.

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