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Literature DB >> 16204567

Sau-PCR, a novel amplification technique for genetic fingerprinting of microorganisms.

Viviana Corich¹, Alessandro Mattiazzi, Elisa Soldati, Angela Carraro, Alessio Giacomini.

Abstract

The proposed technique is based on the digestion of genomic DNA with the restriction endonuclease Sau3AI and subsequent amplification with primers whose core sequence is based on the Sau3AI recognition site. The method was tested on strains of lactic acid bacteria but could be proposed for virtually any culturable organism from which DNA can be extracted.

Entities: Chemical

Mesh：

Substances：

Year: 2005 PMID： 16204567 PMCID： PMC1265919 DOI： 10.1128/AEM.71.10.6401-6406.2005

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

16 in total

1. RAPD-PCR characterization of lactobacilli isolated from artisanal meat plants and traditional fermented sausages of Veneto region (Italy).

Authors: C Andrighetto; L Zampese; A Lombardi
Journal: Lett Appl Microbiol Date: 2001-07 Impact factor: 2.858

2. NEBcutter: A program to cleave DNA with restriction enzymes.

Authors: Tamas Vincze; Janos Posfai; Richard J Roberts
Journal: Nucleic Acids Res Date: 2003-07-01 Impact factor: 16.971

3. AFLP: a new technique for DNA fingerprinting.

Authors: P Vos; R Hogers; M Bleeker; M Reijans; T van de Lee; M Hornes; A Frijters; J Pot; J Peleman; M Kuiper
Journal: Nucleic Acids Res Date: 1995-11-11 Impact factor: 16.971

4. Amplification of anonymous DNA fragments using pairs of long primers generates reproducible DNA fingerprints that are sensitive to genetic variation.

Authors: M Gillings; M Holley
Journal: Electrophoresis Date: 1997-08 Impact factor: 3.535

5. Genetic basis of enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint pattern in Sinorhizobium meliloti and identification of S. meliloti employing PCR primers derived from an ERIC-PCR fragment.

Authors: S Niemann; T Dammann-Kalinowski; A Nagel; A Pühler; W Selbitschka
Journal: Arch Microbiol Date: 1999-07 Impact factor: 2.552

6. Effect of transition interval between melting and annealing temperatures on RAPD analyses.

Authors: M E Schweder; R G Shatters; S H West; R L Smith
Journal: Biotechniques Date: 1995-07 Impact factor: 1.993

7. Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers.

Authors: M Gaudeul; P Taberlet; I Till-Bottraud
Journal: Mol Ecol Date: 2000-10 Impact factor: 6.185

2. Biocontrol Ability and Action Mechanism of Starmerella bacillaris (Synonym Candida zemplinina) Isolated from Wine Musts against Gray Mold Disease Agent Botrytis cinerea on Grape and Their Effects on Alcoholic Fermentation.

Authors: Wilson J Lemos; Barbara Bovo; Chiara Nadai; Giulia Crosato; Milena Carlot; Francesco Favaron; Alessio Giacomini; Viviana Corich
Journal: Front Microbiol Date: 2016-08-15 Impact factor: 5.640

2 in total

Sau-PCR, a novel amplification technique for genetic fingerprinting of microorganisms.

1. RAPD-PCR characterization of lactobacilli isolated from artisanal meat plants and traditional fermented sausages of Veneto region (Italy).

2. NEBcutter: A program to cleave DNA with restriction enzymes.

3. AFLP: a new technique for DNA fingerprinting.

4. Amplification of anonymous DNA fragments using pairs of long primers generates reproducible DNA fingerprints that are sensitive to genetic variation.

5. Genetic basis of enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint pattern in Sinorhizobium meliloti and identification of S. meliloti employing PCR primers derived from an ERIC-PCR fragment.

6. Effect of transition interval between melting and annealing temperatures on RAPD analyses.

7. Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers.

8. Fingerprinting genomes using PCR with arbitrary primers.

9. Studies on transformation of Escherichia coli with plasmids.

10. Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting.

1. Dissemination and Persistence of Pseudomonas spp. in Small-Scale Dairy Farms.

2. Biocontrol Ability and Action Mechanism of Starmerella bacillaris (Synonym Candida zemplinina) Isolated from Wine Musts against Gray Mold Disease Agent Botrytis cinerea on Grape and Their Effects on Alcoholic Fermentation.