BACKGROUND: Oral squamous cell carcinoma (OSCC) is one of the leading cancers in most Asian countries. Alterations of immune function have been detected in OSCC patients. The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) is a central mediator of the immune response involved in a wide range of immuno-inflammatory and infectious diseases. Polymorphism of the TNF-alpha gene has been intensively studied as a potential determinant of susceptibility to numerous cancers. METHODS: We genotyped 192 patients with OSCC and 146 healthy case controls by using polymerase chain reaction-double restriction fragment length polymorphism with amplification-created restriction sites to assess allelic determinants at the TNF-alpha polymorphic sites -308 and -238 in the promoter region. Genotype frequencies were evaluated with Fisher's test. RESULTS: The -308 TNFG (tumor necrosis factor G) allele genotype was higher in patients with OSCC (91.2% vs. 82.2%; P = 0.02) and TNFG/A was lower (8.3% vs. 11.8%; P = 0.02); the -238 TNFG/A allele genotype was lower in patient with OSCC (2.1% vs. 6.9%; P = 0.02). CONCLUSION: This is the first report that the TNF-alpha polymorphism is associated with the risk for OSCC in Taiwan.
BACKGROUND:Oral squamous cell carcinoma (OSCC) is one of the leading cancers in most Asian countries. Alterations of immune function have been detected in OSCC patients. The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) is a central mediator of the immune response involved in a wide range of immuno-inflammatory and infectious diseases. Polymorphism of the TNF-alpha gene has been intensively studied as a potential determinant of susceptibility to numerous cancers. METHODS: We genotyped 192 patients with OSCC and 146 healthy case controls by using polymerase chain reaction-double restriction fragment length polymorphism with amplification-created restriction sites to assess allelic determinants at the TNF-alpha polymorphic sites -308 and -238 in the promoter region. Genotype frequencies were evaluated with Fisher's test. RESULTS: The -308 TNFG (tumor necrosis factor G) allele genotype was higher in patients with OSCC (91.2% vs. 82.2%; P = 0.02) and TNFG/A was lower (8.3% vs. 11.8%; P = 0.02); the -238 TNFG/A allele genotype was lower in patient with OSCC (2.1% vs. 6.9%; P = 0.02). CONCLUSION: This is the first report that the TNF-alpha polymorphism is associated with the risk for OSCC in Taiwan.
Authors: Caiyun Zhang; Erich M Sturgis; Hongliang Zheng; Xicheng Song; Peng Wei; Lei Jin; Li Chao; Qingyi Wei; Guojun Li Journal: Int J Cancer Date: 2013-10-24 Impact factor: 7.396
Authors: Caiyun Zhang; Erich M Sturgis; Hongliang Zheng; Mark E Zafereo; Qingyi Wei; Guojun Li Journal: Int J Cancer Date: 2014-02-27 Impact factor: 7.396
Authors: Lei Jin; Erich M Sturgis; Yang Zhang; Zhigang Huang; Xicheng Song; Chao Li; Qingyi Wei; Guojun Li Journal: Mol Cancer Date: 2013-07-19 Impact factor: 27.401
Authors: Esther Erdei; Li Luo; Huiping Sheng; Erika Maestas; Kirsten A M White; Amanda Mackey; Yan Dong; Marianne Berwick; Douglas E Morse Journal: PLoS One Date: 2013-11-20 Impact factor: 3.240