BACKGROUND: One of the reasons for the large variation in radial strain measured with Doppler strain rate imaging in normal myocardium might be the different strain length (SL) used during analyses. The aim of this study was to evaluate the effect of different SL settings on strain recordings and the method's ability to detect transmural radial strain gradients. METHODS: In 8 anesthetized pigs (mean weight 54 kg) epicardial echocardiography was performed. Strain analysis was carried out by defining the wall as a 1-, 2-, 3-, and 4-layer structure with suitable regions of interest. Peak ejection strain was measured with SL settings of 2 to 14 mm. RESULTS: The systolic (ejection) strain showed large variation with SL. Sampling in one layer gave no significant reduction in strain for increasing SL. The strain in the subepicardial layer was low and decreased when the wall was divided into several layers (15.9 +/- 4.8% [2 layers]-2.1 +/- 2.4% [4 layers]; both measurements with SL = 4 mm). The method could separate 4 layers with SL of 4 mm or less, 3 layers with SL of 6 mm or less, and 2 layers with SL of 8 mm or less. CONCLUSION: When measuring radial strain in the myocardial wall the SL must be low to evaluate transmural strain gradients. With correct settings of SL and region of interest, strain in 4 layers can be distinguished. As a rule of thumb the SL should be set to approximately half the systolic thickness of the wall or half the wall layer.
BACKGROUND: One of the reasons for the large variation in radial strain measured with Doppler strain rate imaging in normal myocardium might be the different strain length (SL) used during analyses. The aim of this study was to evaluate the effect of different SL settings on strain recordings and the method's ability to detect transmural radial strain gradients. METHODS: In 8 anesthetized pigs (mean weight 54 kg) epicardial echocardiography was performed. Strain analysis was carried out by defining the wall as a 1-, 2-, 3-, and 4-layer structure with suitable regions of interest. Peak ejection strain was measured with SL settings of 2 to 14 mm. RESULTS: The systolic (ejection) strain showed large variation with SL. Sampling in one layer gave no significant reduction in strain for increasing SL. The strain in the subepicardial layer was low and decreased when the wall was divided into several layers (15.9 +/- 4.8% [2 layers]-2.1 +/- 2.4% [4 layers]; both measurements with SL = 4 mm). The method could separate 4 layers with SL of 4 mm or less, 3 layers with SL of 6 mm or less, and 2 layers with SL of 8 mm or less. CONCLUSION: When measuring radial strain in the myocardial wall the SL must be low to evaluate transmural strain gradients. With correct settings of SL and region of interest, strain in 4 layers can be distinguished. As a rule of thumb the SL should be set to approximately half the systolic thickness of the wall or half the wall layer.