| Literature DB >> 16198268 |
Michihiko Nakano1, Jun Komatsu, Hirofumi Kurita, Hachiro Yasuda, Shinji Katsura, Akira Mizuno.
Abstract
The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.Entities:
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Year: 2005 PMID： 16198268 DOI： 10.1263/jbb.100.216
Source DB: PubMed Journal: J Biosci Bioeng ISSN： 1347-4421 Impact factor: 2.894