Modeling of lipase-catalyzed acidolysis of sesame oil and caprylic Acid by response surface methodology: optimization of reaction conditions by considering both acyl incorporation and migration.

Lipase-catalyzed acidolysis in hexane to produce structured lipids (SLs) from sesame oil and caprylic acid was optimized by considering both total incorporation (Y1) and acyl migration (Y2). Response surface methodology was applied to model Y1 and Y2, respectively, with three reaction parameters: temperature (X1), reaction time (X2), and substrate molar ratio (X3). Well-fitting models for Y1 and Y2 were established after regression analysis with backward elimination and verified by a chi2 test. All factors investigated positively affected Y1. For Y2, X1 showed the greatest positive effect. However, there was no effect of X3. We predicted the levels of Y2 and acyl incorporation into sn-1,3 positions (Y3) based on Y1. The results showed that over the range of ca. 55 mol % of Y1, Y3 started to decrease, and Y2 increased rapidly, suggesting that Y1 should be kept below ca. 55 mol % to prevent decrease in quality and yield of targeted SLs.