Literature DB >> 16188220

Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution.

Arun K Attri1, Allen P Minton.   

Abstract

A method for rapid detection and quantitative characterization of associations between two proteins in solution is presented, based upon continuous measurement of the Rayleigh light scattering and UV absorbance of a solution whose composition is varied with time in a controlled fashion. Collection and subsequent analysis of data are both rapid and semiautomatic. The results of validation experiments demonstrate that the technique can correctly identify complexes and reliably evaluate equilibrium constants for hetero-association.

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Year:  2005        PMID： 16188220     DOI： 10.1016/j.ab.2005.08.013

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  28 in total

Review 1.  Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.

Authors:  Bram Stynen; Hélène Tournu; Jan Tavernier; Patrick Van Dijck
Journal:  Microbiol Mol Biol Rev       Date:  2012-06       Impact factor: 11.056

2.  Free-solution, label-free protein-protein interactions characterized by dynamic light scattering.

Authors:  Amy D Hanlon; Michael I Larkin; Ryan M Reddick
Journal:  Biophys J       Date:  2010-01-20       Impact factor: 4.033

3.  Rapid quantitative characterization of protein interactions by composition gradient static light scattering.

Authors:  Keiichi Kameyama; Allen P Minton
Journal:  Biophys J       Date:  2005-12-30       Impact factor: 4.033

4.  A high-throughput method for detection of protein self-association and second virial coefficient using size-exclusion chromatography through simultaneous measurement of concentration and scattered light intensity.

Authors:  Harminder Bajaj; Vikas K Sharma; Devendra S Kalonia
Journal:  Pharm Res       Date:  2007-06-19       Impact factor: 4.200

5.  Analysis of self-associating proteins by singular value decomposition of solution scattering data.

Authors:  Tim E Williamson; Bruce A Craig; Elena Kondrashkina; Chris Bailey-Kellogg; Alan M Friedman
Journal:  Biophys J       Date:  2008-01-22       Impact factor: 4.033

6.  Measurement of the second osmotic virial coefficient for protein solutions exhibiting monomer-dimer equilibrium.

Authors:  John R Alford; Brent S Kendrick; John F Carpenter; Theodore W Randolph
Journal:  Anal Biochem       Date:  2008-03-22       Impact factor: 3.365

7.  Quantitative characterization of heparin binding to Tau protein: implication for inducer-mediated Tau filament formation.

Authors:  Hai-Li Zhu; Cristina Fernández; Jun-Bao Fan; Frank Shewmaker; Jie Chen; Allen P Minton; Yi Liang
Journal:  J Biol Chem       Date:  2009-12-03       Impact factor: 5.157

Review 8.  Macromolecular interactions of the bacterial division FtsZ protein: from quantitative biochemistry and crowding to reconstructing minimal divisomes in the test tube.

Authors:  Germán Rivas; Carlos Alfonso; Mercedes Jiménez; Begoña Monterroso; Silvia Zorrilla
Journal:  Biophys Rev       Date:  2013-04-16

9.  Stoichiometries and affinities of interacting proteins from concentration series of solution scattering data: decomposition by least squares and quadratic optimization.

Authors:  Himanshu Chandola; Tim E Williamson; Bruce A Craig; Alan M Friedman; Chris Bailey-Kellogg
Journal:  J Appl Crystallogr       Date:  2014-05-10       Impact factor: 3.304

10.  Fitting two- and three-site binding models to isothermal titration calorimetric data.

Authors:  Chad A Brautigam
Journal:  Methods       Date:  2014-12-05       Impact factor: 3.608
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