Xian-feng Gong1, Min-wei Wang, Takashi Ikejima. 1. China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
Abstract
OBJECTIVE: To study the mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells. METHODS: MTT assay, fluorescence microscopy, DNA agarose gel electrophoresis, flow cytometry and Western blot analysis were carried out to assess the morphological and biochemical changes of A375-S2 cells after capsaicin treatment. RESULTS: Capsaicin induced A375-S2 cell death in a time- and dose-dependent manner. Sub-diploid peak was seen at 24 h after 250 micromol/L capsaicin treatment, and apoptotic bodies and DNA ladder were observed at 36 h after capsaicin treatment. The expression of inhibitor of caspase activated DNase (ICAD) was reduced with the lapse of time. CONCLUSION: Capsaicin induces A375-S2 cell apoptosis and down-regulation of ICAD contributes to this process.
OBJECTIVE: To study the mechanisms of capsaicin-induced apoptosis of humanmelanoma A375-S2 cells. METHODS:MTT assay, fluorescence microscopy, DNA agarose gel electrophoresis, flow cytometry and Western blot analysis were carried out to assess the morphological and biochemical changes of A375-S2 cells after capsaicin treatment. RESULTS:Capsaicin induced A375-S2 cell death in a time- and dose-dependent manner. Sub-diploid peak was seen at 24 h after 250 micromol/L capsaicin treatment, and apoptotic bodies and DNA ladder were observed at 36 h after capsaicin treatment. The expression of inhibitor of caspase activated DNase (ICAD) was reduced with the lapse of time. CONCLUSION:Capsaicin induces A375-S2 cell apoptosis and down-regulation of ICAD contributes to this process.
Authors: Pedro H D M Prazeres; Caroline Leonel; Walison N Silva; Beatriz G S Rocha; Gabryella S P Santos; Alinne C Costa; Caroline C Picoli; Isadora F G Sena; William A Gonçalves; Mariana S Vieira; Pedro A C Costa; Leda M C C Campos; Miriam T P Lopes; Marcos R Costa; Rodrigo R Resende; Thiago M Cunha; Akiva Mintz; Alexander Birbrair Journal: J Cell Mol Med Date: 2020-07-20 Impact factor: 5.310