Literature DB >> 1618645

Analysis of the transcripts of cytochrome P-450IID gene subfamily in bovine adrenal gland.

Y Matsuo1, K Iwahashi, Y Kawai, Y Ichikawa.   

Abstract

An in situ hybridization experiment showed that the gene transcripts of P-450IID subfamily were uniformly distributed in bovine liver cells and at low levels in bovine adrenal cortex and medulla. There was no clear localization of transcripts of P-450IID genes in three adrenocortical zones, zona glomerulosa, zona fasciculata and zona reticularis. The intensity of signal obtained in the adrenal cortex and medulla showed approximately 2.5:1 ratio. The presence of gene transcripts of P-450IID subfamily in adrenocortex was confirmed by amplifying cDNA using PCR and hybridizing the PCR products with P-450IID cDNA.

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Year:  1992        PMID: 1618645     DOI: 10.1007/bf00270032

Source DB:  PubMed          Journal:  Histochemistry        ISSN: 0301-5564


  17 in total

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Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A novel adrenocorticotropin-inducible cytochrome P450 from rat adrenal microsomes catalyzes polycyclic aromatic hydrocarbon metabolism.

Authors:  S Otto; C Marcus; C Pidgeon; C Jefcoate
Journal:  Endocrinology       Date:  1991-08       Impact factor: 4.736

3.  Xenobiotic and endobiotic inhibitors of cytochrome P-450dbl function, the target of the debrisoquine/sparteine type polymorphism.

Authors:  R Fonne-Pfister; U A Meyer
Journal:  Biochem Pharmacol       Date:  1988-10-15       Impact factor: 5.858

4.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

Review 5.  The molecular biology of cytochrome P450s.

Authors:  F J Gonzalez
Journal:  Pharmacol Rev       Date:  1988-12       Impact factor: 25.468

6.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

7.  Relation of canrenone to the actions of spironolactone on adrenal cytochrome P-450 dependent enzymes.

Authors:  J W Greiner; R C Rumbaugh; R E Kramer; H D Colby
Journal:  Endocrinology       Date:  1978-10       Impact factor: 4.736

8.  Purification and characterization of the human liver cytochromes P-450 involved in debrisoquine 4-hydroxylation and phenacetin O-deethylation, two prototypes for genetic polymorphism in oxidative drug metabolism.

Authors:  L M Distlerath; P E Reilly; M V Martin; G G Davis; G R Wilkinson; F P Guengerich
Journal:  J Biol Chem       Date:  1985-07-25       Impact factor: 5.157

9.  Debrisoquine/sparteine-type polymorphism of drug oxidation. Purification and characterization of two functionally different human liver cytochrome P-450 isozymes involved in impaired hydroxylation of the prototype substrate bufuralol.

Authors:  J Gut; T Catin; P Dayer; T Kronbach; U Zanger; U A Meyer
Journal:  J Biol Chem       Date:  1986-09-05       Impact factor: 5.157

10.  Debrisoquine 4-hydroxylase: characterization of a new P450 gene subfamily, regulation, chromosomal mapping, and molecular analysis of the DA rat polymorphism.

Authors:  F J Gonzalez; T Matsunaga; K Nagata; U A Meyer; D W Nebert; J Pastewka; C A Kozak; J Gillette; H V Gelboin; J P Hardwick
Journal:  DNA       Date:  1987-04
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