| Literature DB >> 16186245 |
Sophie Haupt1,2, Tanya Stroganova2, Eugene Ryabov3, Sang Hyon Kim2, Gill Fraser2, George Duncan2, Mike A Mayo2, Hugh Barker2, Michael Taliansky2.
Abstract
Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an Agrobacterium vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting [the nucleolar localization signal (NoLS)]. Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from Agrobacterium. However, P5-GFP lost its nucleolar localization in the presence of replicating PLRV.Entities:
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Year: 2005 PMID： 16186245 DOI： 10.1099/vir.0.81101-0
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891