Literature DB >> 16182385

Indirect sandwich ELISA for antigen detection of African swine fever virus: comparison of polyclonal and monoclonal antibodies.

Geoffrey H Hutchings1, Nigel P Ferris.   

Abstract

Two indirect, sandwich ELISAs are described for use in African swine fever (ASF) diagnosis. One assay uses polyclonal serum raised in rabbits and guinea pigs against the cytoplasmic soluble ASF virus protein and the second, a combination of monoclonal antibody raised against the VP73 protein and rabbit polyclonal serum. Both assays have been shown to detect antigen of representative field strains of phylogenetically distinct groupings of ASF virus but the ELISA, which utilises polyclonal antisera was slightly more sensitive than that using the monoclonal antibody.

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Year:  2005        PMID： 16182385     DOI： 10.1016/j.jviromet.2005.08.009

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  8 in total

1.  HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies.

Authors:  Huijun Zhao; Jiahui Ren; Shuya Wu; Haoran Guo; Yongkun Du; Bo Wan; Pengchao Ji; Yanan Wu; Guoqing Zhuang; Angke Zhang; Gaiping Zhang
Journal:  Appl Microbiol Biotechnol       Date:  2022-05-25       Impact factor: 5.560

2.  Development of a Potential Penside Colorimetric LAMP Assay Using Neutral Red for Detection of African Swine Fever Virus.

Authors:  Yang Wang; Junfei Dai; Yongsheng Liu; Jifei Yang; Qian Hou; Yunwen Ou; Yaozhong Ding; Bing Ma; Haotai Chen; MiaoMiao Li; Yuefeng Sun; Haixue Zheng; Keshan Zhang; Ashenafi Kiros Wubshet; Alexei D Zaberezhny; Taras I Aliper; Kazimierz Tarasiuk; Zygmunt Pejsak; Zhijie Liu; Yongguang Zhang; Jie Zhang
Journal:  Front Microbiol       Date:  2021-04-23       Impact factor: 5.640

3.  Development of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus Antigen.

Authors:  Wei Wang; Jizong Li; Baochao Fan; Xuehan Zhang; Rongli Guo; Yongxiang Zhao; Junming Zhou; Jinzhu Zhou; Dongbo Sun; Bin Li
Journal:  Viruses       Date:  2021-11-30       Impact factor: 5.048

Review 4.  Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management.

Authors:  Dexin Zeng; Bingxu Qian; Yunfei Li; Kai Zong; Wanqing Peng; Kai Liao; Xiaofeng Yu; Juanjuan Sun; Xiaying Lv; Liu Ding; Manman Wang; Tingting Zhou; Yuan Jiang; Jinming Li; Feng Xue; Xiaodong Wu; Jianjun Dai
Journal:  Front Microbiol       Date:  2022-09-30       Impact factor: 6.064

5.  A New Method for Detection African Swine Fever Virus: Time-resolved Fluorescence Immunoassay.

Authors:  Cuicui Chen; Hongrui Lai; Huankun Liang; Ying He; Guiling Guo; Laiqing Li
Journal:  J Fluoresc       Date:  2021-06-01       Impact factor: 2.217

6.  African swine fever virus isolate, Georgia, 2007.

Authors:  Rebecca J Rowlands; Vincent Michaud; Livio Heath; Geoff Hutchings; Chris Oura; Wilna Vosloo; Rahana Dwarka; Tinatin Onashvili; Emmanuel Albina; Linda K Dixon
Journal:  Emerg Infect Dis       Date:  2008-12       Impact factor: 6.883

7.  Prevalence of African swine fever virus in apparently healthy domestic pigs in Uganda.

Authors:  David Kalenzi Atuhaire; Mathias Afayoa; Sylvester Ochwo; Savannah Mwesigwa; Frank Norbert Mwiine; Julius Boniface Okuni; William Olaho-Mukani; Lonzy Ojok
Journal:  BMC Vet Res       Date:  2013-12-26       Impact factor: 2.741

8.  Development of a double-recombinant antibody sandwich ELISA for quantitative detection of epsilon toxoid concentration in inactivated Clostridium perfringens vaccines.

Authors:  Maryam Alibeiki; Mehdi Golchin; Mohammad Tabatabaei
Journal:  BMC Vet Res       Date:  2020-09-29       Impact factor: 2.741
  8 in total

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