W D Cardona-Maya1, A P Cadavid. 1. Grupo Reproducción-Biogénesis, Universidad de Antioquia, Medellín, Colombia. wdcmaya@yahoo.com
Abstract
OBJECTIVE: The aim of this investigation was to evaluate the role of the monosaccharides, mannose and N-acetylglucosamine in the induction of the acrosome reaction in human spermatozoa. METHODS: Each individual gave a semen sample, the samples were capacitated using bovien serum albumin; the analysis of the mannose and N-acetylglucosamine induced acrosome reaction was evaluated for fluorescence microscopy and flow cytometry. RESULTS: The results obtained in the acrosomal reaction in the 10 samples evaluated by flow cytometry showed that only calcium ionophore A23187 induced acrosomal reaction (p < 0.001); in contrast, by fluorescence microscopy, was observed a statistically significant increase in the induced acrosomal reaction with mannose and N-acetylglucosamine (p < 0.001). CONCLUSIONS: The monosaccharides mannose and N-acetylglucosamine induces partial acrosomal reaction and it does not complete, therefore the spermatozoa due to partial acrosomal reaction and the morphologics modifications that suffer during that process is able to fertilize the oocyte.
OBJECTIVE: The aim of this investigation was to evaluate the role of the monosaccharides, mannose and N-acetylglucosamine in the induction of the acrosome reaction in human spermatozoa. METHODS: Each individual gave a semen sample, the samples were capacitated using bovien serum albumin; the analysis of the mannose and N-acetylglucosamine induced acrosome reaction was evaluated for fluorescence microscopy and flow cytometry. RESULTS: The results obtained in the acrosomal reaction in the 10 samples evaluated by flow cytometry showed that only calcium ionophore A23187 induced acrosomal reaction (p < 0.001); in contrast, by fluorescence microscopy, was observed a statistically significant increase in the induced acrosomal reaction with mannose and N-acetylglucosamine (p < 0.001). CONCLUSIONS: The monosaccharidesmannose and N-acetylglucosamine induces partial acrosomal reaction and it does not complete, therefore the spermatozoa due to partial acrosomal reaction and the morphologics modifications that suffer during that process is able to fertilize the oocyte.