| Literature DB >> 16179161 |
Atsuo Inoue1, Kenji A Takahashi, Osam Mazda, Ryu Terauchi, Yuji Arai, Tsunao Kishida, Masaharu Shin-Ya, Hidetsugu Asada, Toru Morihara, Hitoshi Tonomura, Suzuyo Ohashi, Yoshiteru Kajikawa, Yutaka Kawahito, Jiro Imanishi, Mitsuhiro Kawata, Toshikazu Kubo.
Abstract
RNA interference provides the powerful means of sequence-specific gene silencing. Particularly, small interfering RNA (siRNA) duplexes may be potentially useful for therapeutic molecular targeting of human diseases, although novel delivery systems should be devised to achieve efficient and organ-specific transduction of siRNA. In the present study, we demonstrated that electro-transfer of a siRNA-polyamine complex made efficient and specific gene knockdown possible in the articular synovium. Targeted suppression of the tumor necrosis factor-alpha gene through this procedure significantly ameliorated collagen-induced arthritis in rats. Our results suggest the potential feasibility of therapeutic intervention with RNA medicines for treatment of rheumatoid and other locomotor diseases.Entities:
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Year: 2005 PMID： 16179161 DOI： 10.1016/j.bbrc.2005.08.198
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575