| Literature DB >> 1617733 |
L Chevalet1, G Tiraby, B Cabane, G Loison.
Abstract
A transformation procedure based on the complementation of a genetic defect was developed using a nitrate reductase-deficient mutant of Aspergillus flavus. The initial transformation efficiency was improved 40-fold by combining factors in a planned experimental program. Although low, this transformation rate was sufficient to obtain transformants in which the urate oxidase-encoding gene (uaZ) was disrupted in a gene replacement experiment. These new uaZ- strains were unable to utilize uric acid as the unique nitrogen source and could be reversed directly to the wild-type phenotype in second order transformation experiments using a urate oxidase-expressing vector.Entities:
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Year: 1992 PMID： 1617733 DOI： 10.1007/bf00351654
Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886