Literature DB >> 16177019

Resazurin assay of radiation response in cultured cells.

S Anoopkumar-Dukie1, J B Carey, T Conere, E O'sullivan, F N van Pelt, A Allshire.   

Abstract

We describe use of resazurin reduction for measurement of cell response to irradiation as a simple and non-destructive assay that complements the conventional colony forming assay and can readily be applied to both adherent and non-adherent cell cultures. The resazurin method yields data comparable with the colony forming assay as well as to assay of DNA synthesis (BrdU incorporation), giving an OER (oxygen enhancement ratio) of 2.5 at 60% isoeffect level versus 3.1 for the colony forming assay. Intraday and interday precisions for the resazurin assay were 4.1% and 5.2%, respectively.

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Year:  2005        PMID： 16177019     DOI： 10.1259/bjr/54004230

Source DB:  PubMed          Journal:  Br J Radiol        ISSN： 0007-1285            Impact factor:   3.039


  38 in total

1.  Radiation pharmacogenomics: a genome-wide association approach to identify radiation response biomarkers using human lymphoblastoid cell lines.

Authors:  Nifang Niu; Yuxin Qin; Brooke L Fridley; Junmei Hou; Krishna R Kalari; Minjia Zhu; Tse-Yu Wu; Gregory D Jenkins; Anthony Batzler; Liewei Wang
Journal:  Genome Res       Date:  2010-10-05       Impact factor: 9.043

2.  The mitochondrial translocator protein, TSPO, inhibits HIV-1 envelope glycoprotein biosynthesis via the endoplasmic reticulum-associated protein degradation pathway.

Authors:  Tao Zhou; Ying Dang; Yong-Hui Zheng
Journal:  J Virol       Date:  2014-01-08       Impact factor: 5.103

3.  NCTR25 fusion facilitates the formation of TRAIL polymers that selectively activate TRAIL receptors with higher potency and efficacy than TRAIL.

Authors:  Yan Wang; Qiong Lei; Cangjie Shen; Nan Wang
Journal:  Cancer Chemother Pharmacol       Date:  2021-05-04       Impact factor: 3.333

4.  In vitro efficacy of dicationic compounds and mefloquine enantiomers against Echinococcus multilocularis metacestodes.

Authors:  Britta Stadelmann; Tatiana Küster; Sabrina Scholl; Fabienne Barna; Christian Kropf; Jennifer Keiser; David W Boykin; Chad E Stephens; Andrew Hemphill
Journal:  Antimicrob Agents Chemother       Date:  2011-07-18       Impact factor: 5.191

5.  Transthyretin deposition in articular cartilage: a novel mechanism in the pathogenesis of osteoarthritis.

Authors:  Yukio Akasaki; Natàlia Reixach; Tokio Matsuzaki; Oscar Alvarez-Garcia; Merissa Olmer; Yukihide Iwamoto; Joel N Buxbaum; Martin K Lotz
Journal:  Arthritis Rheumatol       Date:  2015-05       Impact factor: 10.995

6.  Detection of oxidative stress-induced carbonylation in live mammalian cells.

Authors:  Kamalika Mukherjee; Tak Ian Chio; Dan L Sackett; Susan L Bane
Journal:  Free Radic Biol Med       Date:  2015-03-20       Impact factor: 7.376

7.  6-Methoxyquinoline complexes as lung carcinoma agents: induction of oxidative damage on A549 monolayer and multicellular spheroid model.

Authors:  J F Cadavid-Vargas; C Villa-Pérez; M C Ruiz; I E León; G C Valencia-Uribe; D B Soria; S B Etcheverry; A L Di Virgilio
Journal:  J Biol Inorg Chem       Date:  2019-01-30       Impact factor: 3.358

8.  Meprinα transactivates the epidermal growth factor receptor (EGFR) via ligand shedding, thereby enhancing colorectal cancer cell proliferation and migration.

Authors:  Petra Minder; Elke Bayha; Christoph Becker-Pauly; Erwin E Sterchi
Journal:  J Biol Chem       Date:  2012-08-24       Impact factor: 5.157

9.  Streptomycin-starved Mycobacterium tuberculosis 18b, a drug discovery tool for latent tuberculosis.

Authors:  Ming Zhang; Claudia Sala; Ruben C Hartkoorn; Neeraj Dhar; Alfonso Mendoza-Losana; Stewart T Cole
Journal:  Antimicrob Agents Chemother       Date:  2012-08-27       Impact factor: 5.191

10.  The Chemical Molecule B355252 is Neuroprotective in an In Vitro Model of Parkinson's Disease.

Authors:  Nailya S Gliyazova; Gordon C Ibeanu
Journal:  Cell Mol Neurobiol       Date:  2015-12-09       Impact factor: 5.046
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