Literature DB >> 16174438

Assessing phylogenetic relationships of Lycium samples using RAPD and entropy theory.

Xiao-lin Yin1, Kai-tai Fang, Yi-zeng Liang, Ricky N S Wong, Amber W Y Ha.   

Abstract

AIM: To evaluate the phylogenetic relationships among related species of Lycium samples.
METHODS: Random amplified polymorphic DNA (RAPD) fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints.
RESULTS: Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information.
CONCLUSION: Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.

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Substances:

Year:  2005        PMID: 16174438     DOI: 10.1111/j.1745-7254.2005.00197.x

Source DB:  PubMed          Journal:  Acta Pharmacol Sin        ISSN: 1671-4083            Impact factor:   6.150


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