Literature DB >> 16170463

Application of cryofixation and cryoultramicrotomy for biological electron microscopy.

Kenji Saga1.   

Abstract

Conventional chemical fixation and embedding of specimens in resins are accompanied by many artifacts, including postmortem structural alterations. Antigenicity of constituents of specimens can be deteriorated and soluble elements relocated in the process of chemical fixation and resin-embedding. Cryofixation and cryoultramicrotomy will overcome many of these drawbacks of chemical fixation and resin embedding. The theoretical background, equipment, methods, and applications of cryofixation and cryoultramicrotomy for biological specimens are reviewed.

Mesh：

Year:  2005        PMID： 16170463     DOI： 10.1007/s00795-005-0287-2

Source DB:  PubMed          Journal:  Med Mol Morphol        ISSN： 1860-1499            Impact factor:   2.309


  29 in total

Review 1.  High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling.

Authors:  K McDonald
Journal:  Methods Mol Biol       Date:  1999

2.  A cryotransmission electron microscopy study of skin barrier formation.

Authors:  Lars Norlén; Ashraf Al-Amoudi; Jaques Dubochet
Journal:  J Invest Dermatol       Date:  2003-04       Impact factor: 8.551

Review 3.  Improving structural integrity of cryosections for immunogold labeling.

Authors:  W Liou; H J Geuze; J W Slot
Journal:  Histochem Cell Biol       Date:  1996-07       Impact factor: 4.304

4.  High pressure freezing comes of age.

Authors:  D Studer; M Michel; M Müller
Journal:  Scanning Microsc Suppl       Date:  1989

5.  Cutting artefacts on ultrathin cryosections of biological bulk specimens.

Authors:  K Richter
Journal:  Micron       Date:  1994       Impact factor: 2.251

6.  Cryo-electron microscopy of vitrified insect flight muscle.

Authors:  A W McDowall; W Hofmann; J Lepault; M Adrian; J Dubochet
Journal:  J Mol Biol       Date:  1984-09-05       Impact factor: 5.469

Review 7.  Structure and function of human sweat glands studied with histochemistry and cytochemistry.

Authors:  Kenji Saga
Journal:  Prog Histochem Cytochem       Date:  2002

8.  Electron probe X-ray microanalysis of cellular ions in the eccrine secretory coil cells during methacholine stimulation.

Authors:  K Saga; K Sato
Journal:  J Membr Biol       Date:  1989-01       Impact factor: 1.843

9.  Elemental distribution in striated muscle and the effects of hypertonicity. Electron probe analysis of cryo sections.

Authors:  A V Somlyo; H Shuman; A P Somlyo
Journal:  J Cell Biol       Date:  1977-09       Impact factor: 10.539

10.  A technique for ultracryotomy of cell suspensions and tissues.

Authors:  K T Tokuyasu
Journal:  J Cell Biol       Date:  1973-05       Impact factor: 10.539
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  2 in total

1.  Morphological analysis of lamellar structures in mouse type II pneumocytes by quick-freezing and freeze-drying with osmium tetroxide vapor-fixation.

Authors:  Chunlu Yang; Nobuo Terada; Nobuhiko Ohno; Yasuhisa Fujii; Shinichi Ohno
Journal:  Med Mol Morphol       Date:  2006-06       Impact factor: 2.309

2.  Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometry.

Authors:  Hiroshi Ageta; Sayaka Asai; Yuki Sugiura; Naoko Goto-Inoue; Nobuhiro Zaima; Mitsutoshi Setou
Journal:  Med Mol Morphol       Date:  2009-03-18       Impact factor: 2.309
  2 in total

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