BACKGROUND: A new basis for diagnostic tests is being provided by the vast amount of data on gene expression that are now becoming available through large-scale measurement of mRNA abundance. The insights gained from these resources are most likely going to provide both a better basic understanding of disease mechanisms, and to identify molecular markers for more precise diagnoses and for prediction of prognosis and treatment response. METHODS: Some quantitative RT-PCR assays are utilized today for diagnosis of both malignant and non-malignant disease, but the use of gene expression measurements in clinical medicine can be expected to increase dramatically. CONCLUSIONS: There are important technical issues that must be adequately solved in order to obtain robust assays, such as standardized protocols with appropriate quality controls that ensure reliable data for the specific samples being analysed and good inter-laboratory reproducibility.
BACKGROUND: A new basis for diagnostic tests is being provided by the vast amount of data on gene expression that are now becoming available through large-scale measurement of mRNA abundance. The insights gained from these resources are most likely going to provide both a better basic understanding of disease mechanisms, and to identify molecular markers for more precise diagnoses and for prediction of prognosis and treatment response. METHODS: Some quantitative RT-PCR assays are utilized today for diagnosis of both malignant and non-malignant disease, but the use of gene expression measurements in clinical medicine can be expected to increase dramatically. CONCLUSIONS: There are important technical issues that must be adequately solved in order to obtain robust assays, such as standardized protocols with appropriate quality controls that ensure reliable data for the specific samples being analysed and good inter-laboratory reproducibility.
Authors: Josiah N Orina; Anna Maria Calcagno; Chung-Pu Wu; Sudhir Varma; Joanna Shih; Min Lin; Gabriel Eichler; John N Weinstein; Yves Pommier; Suresh V Ambudkar; Michael M Gottesman; Jean-Pierre Gillet Journal: Mol Cancer Ther Date: 2009-07-07 Impact factor: 6.261
Authors: Janyaporn Phuchareon; Annemieke van Zante; Jonathan B Overdevest; Frank McCormick; David W Eisele; Osamu Tetsu Journal: Transl Oncol Date: 2014-10-24 Impact factor: 4.243
Authors: Janyaporn Phuchareon; Jonathan B Overdevest; Frank McCormick; David W Eisele; Annemieke van Zante; Osamu Tetsu Journal: Transl Oncol Date: 2014-12 Impact factor: 4.243