Literature DB >> 16168616

Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay.

Manoj Kumar1, Inshad Ali Khan, Vijashoor Verma, Narender Kalyan, Giulam Nabi Qazi.   

Abstract

A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16168616     DOI： 10.1016/j.diagmicrobio.2005.05.011

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  9 in total

1.  Application of a microcalorimetric method for determining drug susceptibility in mycobacterium species.

Authors:  M Howell; D Wirz; A U Daniels; O Braissant
Journal:  J Clin Microbiol       Date:  2011-11-16       Impact factor: 5.948

2.  Antimycobacterial compound of chitosan and ethambutol: ultrastructural biological evaluation in vitro against Mycobacterium tuberculosis.

Authors:  M E F A G Oliveira; Y J A Silva; L A Azevedo; L A Linhares; L M L Montenegro; S Alves; R V S Amorim
Journal:  Appl Microbiol Biotechnol       Date:  2021-11-29       Impact factor: 4.813

Review 3.  Nitrate reductase assay for rapid detection of isoniazid, rifampin, ethambutol, and streptomycin resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis.

Authors:  Ahmet Yilmaz Coban; Aydin Deveci; Ahmet Tevfik Sunter; Anandi Martin
Journal:  J Clin Microbiol       Date:  2013-10-16       Impact factor: 5.948

4.  Non-commercial phenotypic assays for the detection of Mycobacterium tuberculosis drug resistance: a systematic review.

Authors:  Irina Kontsevaya; Jim Werngren; Yen Holicka; Kadri Klaos; Anh Tran; Vladyslav Nikolayevskyy
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2019-10-30       Impact factor: 3.267

5.  Evaluation of a novel biphasic culture medium for recovery of mycobacteria: a multi-center study.

Authors:  Zhenling Cui; Jie Wang; Changtai Zhu; Xiaochen Huang; Junmei Lu; Qing Wang; Zhongnan Chen; Junling Wang; Yan Zhang; Delin Gu; Lingjie Jing; Jin Chen; Ruijuan Zheng; Lianhua Qin; Hua Yang; Ruiliang Jin; Zhonghua Liu; Aixiao Bi; Jinming Liu; Zhongyi Hu
Journal:  PLoS One       Date:  2012-04-27       Impact factor: 3.240

6.  Association of mutation patterns in gyrA/B genes and ofloxacin resistance levels in Mycobacterium tuberculosis isolates from East China in 2009.

Authors:  Zhenling Cui; Jie Wang; Junmei Lu; Xiaochen Huang; Zhongyi Hu
Journal:  BMC Infect Dis       Date:  2011-03-29       Impact factor: 3.090

7.  Evaluation of the performance of nitrate reductase assay for rapid drug-susceptibility testing of mycobacterium tuberculosis in north India.

Authors:  Anamika Gupta; Malay Ranjan Sen; Tribhuban Mohan Mohapatra; Shampa Anupurba
Journal:  J Health Popul Nutr       Date:  2011-02       Impact factor: 2.000

8.  Nitrate reductase assay using sodium nitrate for rapid detection of multidrug resistant tuberculosis.

Authors:  Maíra Bidart Macedo; Andrea Von Groll; Krista Fissette; Juan Carlos Palomino; Pedro Eduardo Almeida da Silva; Anandi Martin
Journal:  Braz J Microbiol       Date:  2012-06-01       Impact factor: 2.476

9.  A deletion in the RD105 region confers resistance to multiple drugs in Mycobacterium tuberculosis.

Authors:  Lianhua Qin; Jie Wang; Junmei Lu; Hua Yang; Ruijuan Zheng; Zhonghua Liu; Xiaochen Huang; Yonghong Feng; Zhongyi Hu; Baoxue Ge
Journal:  BMC Biol       Date:  2019-01-25       Impact factor: 7.431
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.