Determination of morphine by molecular imprinting-chemiluminescence method.

A molecular imprinted polymer of morphine was synthesized. Using the imprinted polymer as the recognition material, sodium sulfite as the protective agent, and a potassium permanganate-morphine chemiluminescence system as the detection system, a molecular imprinting-chemiluminescence method for the determination of morphine was established. The linear response range of this method was 5.0 x 10-9-1.0 x 10-6 g/mL (r = 0.9981) and the detection limit was 2 x 10-9 g/mL. The coefficient of variation for 1.0 x 10-7 g/mL morphine solution was 2.8% (n = 9). This method was applied to the determination of morphine in the urine of the heroin abusers with satisfactory results.