Literature DB >> 1616700

Loading and transfer control for Northern hybridization.

R Correa-Rotter1, C N Mariash, M E Rosenberg.   

Abstract

We report a simple and inexpensive method to quantitate loading and transfer of RNA and to examine RNA integrity for use with Northern hybridization. This technique involves quantitation by computer-assisted video densitometry of a negative photograph of 28S and 18S rRNA from ethidium bromide-stained RNA fixed to nylon membranes. This method eliminates the issue of variability of expression of "housekeeping" genes and the need for a second round of hybridization to quantitate control probes.

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Year:  1992        PMID： 1616700

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  21 in total

1.  Usher syndrome 1D and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of the novel cadherin-like gene CDH23.

Authors:  J M Bork; L M Peters; S Riazuddin; S L Bernstein; Z M Ahmed; S L Ness; R Polomeno; A Ramesh; M Schloss; C R Srisailpathy; S Wayne; S Bellman; D Desmukh; Z Ahmed; S N Khan; V M Kaloustian; X C Li; A Lalwani; S Riazuddin; M Bitner-Glindzicz; W E Nance; X Z Liu; G Wistow; R J Smith; A J Griffith; E R Wilcox; T B Friedman; R J Morell
Journal:  Am J Hum Genet       Date:  2000-11-21       Impact factor: 11.025

2.  Mutations of the protocadherin gene PCDH15 cause Usher syndrome type 1F.

Authors:  Z M Ahmed; S Riazuddin; S L Bernstein; Z Ahmed; S Khan; A J Griffith; R J Morell; T B Friedman; S Riazuddin; E R Wilcox
Journal:  Am J Hum Genet       Date:  2001-06-07       Impact factor: 11.025

3.  Expression of the estrogen receptor gene in developing and adult human breast.

Authors:  M Boyd; R H Hildebrandt; S A Bartow
Journal:  Breast Cancer Res Treat       Date:  1996       Impact factor: 4.872

4.  Immunosuppressant FK506 enhances phosphorylation of nitric oxide synthase and protects against glutamate neurotoxicity.

Authors:  T M Dawson; J P Steiner; V L Dawson; J L Dinerman; G R Uhl; S H Snyder
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205

5.  Regulation of mRNA transcripts and DNA synthesis in the rat heart following intravenous injection of transforming growth factor beta 1.

Authors:  A Sigel; J A Douglas; M Eghbali-Webb
Journal:  Mol Cell Biochem       Date:  1994-12-21       Impact factor: 3.396

6.  Estradiol enhances prostaglandin E2 receptor gene expression in luteinizing hormone-releasing hormone (LHRH) neurons and facilitates the LHRH response to PGE2 by activating a glia-to-neuron signaling pathway.

Authors:  F Rage; B J Lee; Y J Ma; S R Ojeda
Journal:  J Neurosci       Date:  1997-12-01       Impact factor: 6.167

7.  Isolation and characterization of a mutant of Saccharomyces cerevisiae with pleiotropic deficiencies in transcriptional activation and repression.

Authors:  E Lamping; J Lückl; F Paltauf; S A Henry; S D Kohlwein
Journal:  Genetics       Date:  1994-05       Impact factor: 4.562

8.  Systemic short-chain fatty acids rapidly alter gastrointestinal structure, function, and expression of early response genes.

Authors:  K A Tappenden; M I McBurney
Journal:  Dig Dis Sci       Date:  1998-07       Impact factor: 3.199

9.  Nucleotide sequence of the genomic region encompassing Adh and Adh-dup genes of D. lebanonensis (Scaptodrosophila): gene expression and evolutionary relationships.

Authors:  E Juan; M Papaceit; A Quintana
Journal:  J Mol Evol       Date:  1994-05       Impact factor: 2.395

10.  Variable regulation by insulin of insulin gene expression in HIT-T15 cells.

Authors:  H J Zhang; B Petersen; R P Robertson
Journal:  Diabetologia       Date:  1994-06       Impact factor: 10.122
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