| Literature DB >> 16166527 |
Kentaro Hayashi1, Taku Ohsawa, Kazuo Kobayashi, Naotake Ogasawara, Mitsuo Ogura.
Abstract
srfA is an operon required for the synthesis of surfactin and the development of genetic competence in Bacillus subtilis. We observed that the expression of srfA is downregulated upon treatment with H2O2. Thus, we examined the involvement of several oxidative stress-responsive transcription factors in srfA expression. Our DNA microarray analysis revealed that the H2O2 stress-responsive regulator PerR is required for srfA expression. This was confirmed by lacZ fusion analysis. A ComX feeding assay and epistatic analyses revealed that the role of PerR in srfA expression is independent of other known regulators of srfA expression, namely, comQXP, rapC, and spx. Gel mobility shift and footprint assays revealed that PerR binds directly to two tandemly arranged noncanonical PerR boxes located in the upstream promoter region of srfA. A transcriptional srfA-lacZ fusion lacking both PerR boxes showed diminished and PerR-independent expression, indicating that the PerR boxes we identified function as positive cis elements for srfA transcription.Entities:
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Year: 2005 PMID： 16166527 PMCID： PMC1251593 DOI： 10.1128/JB.187.19.6659-6667.2005
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490