Literature DB >> 16163485

Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis.

Angela Scipioni1, Stefania Stefanini, Rocco Santone, Mauro Giorgi.   

Abstract

Expression of phosphodiesterase 5 (PDE5) in the rat testis at several pre and postnatal developmental stages was investigated by immunohistochemistry. The enzyme was localised in vascular smooth muscle cells, as well as in Leydig and peritubular cells. The latter were identified as myoid, based on their immunoreactivity to desmin and alpha-smooth muscle actin. The presence of PDE5 in myoid cells was confirmed by Western blot analysis and immunohistochemistry performed on highly purified cell fractions, obtained from 16-day-old rats. The expression of PDE5 in these somatic cells of rat testis is discussed in view of the roles played by cGMP signal transduction pathways in the mammalian male reproductive function.

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Year:  2005        PMID： 16163485     DOI： 10.1007/s00418-005-0057-1

Source DB:  PubMed          Journal:  Histochem Cell Biol        ISSN： 0948-6143            Impact factor:   4.304


  41 in total

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Journal:  J Histochem Cytochem       Date:  2000-05       Impact factor: 2.479

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  10 in total

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Authors:  Li-Chun Lisa Tsai; Joseph A Beavo
Journal:  Curr Opin Pharmacol       Date:  2011-09-29       Impact factor: 5.547

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Journal:  Histochem Cell Biol       Date:  2005-11-11       Impact factor: 4.304

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Journal:  Histochem Cell Biol       Date:  2006-11-24       Impact factor: 4.304

4.  Tadalafil modulates aromatase activity and androgen receptor expression in a human osteoblastic cell in vitro model.

Authors:  A Aversa; S Fittipaldi; V M Bimonte; F Wannenes; V Papa; D Francomano; E A Greco; A Lenzi; S Migliaccio
Journal:  J Endocrinol Invest       Date:  2015-07-02       Impact factor: 4.256

5.  Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion.

Authors:  Karina Lidianne Alcântara Saraiva; Amanda Karolina Soares E Silva; Maria Inês Wanderley; Araken Almeida De Araújo; José Roberto Botelho De Souza; Christina Alves Peixoto
Journal:  Int J Exp Pathol       Date:  2009-08       Impact factor: 1.925

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Authors:  Michelle B Henesy; Andrea L Britain; Bing Zhu; Lauren Amable; Richard E Honkanen; Jackie D Corbin; Sharron H Francis; Thomas C Rich
Journal:  PLoS One       Date:  2012-08-02       Impact factor: 3.240

8.  Effect of vardenafil on semen parameters in infertile men: a pilot study evaluating short-term treatment.

Authors:  R Rago; P Salacone; L Caponecchia; I Marcucci; C Fiori; A Sebastianelli
Journal:  J Endocrinol Invest       Date:  2012-04-18       Impact factor: 5.467

9.  Aphrodisiac Activity of Eulophia macrobulbon Extract on Erectile Dysfunction in Male Aged Rats.

Authors:  Watcharaporn Preedapirom; Kanokwan Changwichit; Piyarat Srisawang; Kornkanok Ingkaninan; Pornnarin Taepavarapruk
Journal:  Biomed Res Int       Date:  2018-07-09       Impact factor: 3.411

10.  Correcting imbalance of sex hormones by a phosphodiesterase 5 inhibitor improves copulatory dysfunction in male rats with type 2 diabetes.

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Journal:  BMJ Open Diabetes Res Care       Date:  2020-05
  10 in total

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