Literature DB >> 16161178

Examination of dye-protein interaction by gel-permeation chromatography.

Jolanta Sereikaite1, Vladas-Algirdas Bumelis.   

Abstract

The interaction of Cibacron blue F3G-A with two therapeutic proteins, recombinant human growth hormone and recombinant human interferon-alpha2b, has been examined by applying gel-permeation chromatography in combination with the absorption difference spectroscopy. The complexes of these proteins with Cibacron blue F3G-A have been isolated, and their absorbance spectra have been registered. The influence of Cibacron blue F3G-A on the oligomeric state of proteins has been investigated. It was found that Cibacron blue F3G-A promotes the generation of interferon-alpha2b dimers at pH 5.0. Copyright (c) 2005 John Wiley & Sons, Ltd.

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Year:  2006        PMID： 16161178     DOI： 10.1002/bmc.552

Source DB:  PubMed          Journal:  Biomed Chromatogr        ISSN： 0269-3879            Impact factor:   1.902


  5 in total

Review 1.  Nanoparticle technology: amplifying the effective sensitivity of biomarker detection to create a urine test for hGH.

Authors:  Claudia Fredolini; Davide Tamburro; Guido Gambara; Benjamin S Lepene; Virginia Espina; Emanuel F Petricoin; Lance A Liotta; Alessandra Luchini
Journal:  Drug Test Anal       Date:  2009-09       Impact factor: 3.345

2.  Nanoparticle technology: Addressing the fundamental roadblocks to protein biomarker discovery.

Authors:  Alessandra Luchini; Caterina Longo; Virginia Espina; Emanuel F Petricoin; Lance A Liotta
Journal:  J Mater Chem       Date:  2009-08-07

3.  Concentration and Preservation of Very Low Abundance Biomarkers in Urine, such as Human Growth Hormone (hGH), by Cibacron Blue F3G-A Loaded Hydrogel Particles.

Authors:  Claudia Fredolini; Francesco Meani; K Alex Reeder; Sally Rucker; Alexis Patanarut; Palma J Botterell; Barney Bishop; Caterina Longo; Virginia Espina; Emanuel F Petricoin; Lance A Liotta; Alessandra Luchini
Journal:  Nano Res       Date:  2008-12       Impact factor: 8.897

4.  Chemokine-Releasing Nanoparticles for Manipulation of Lymph Node Microenvironment.

Authors:  Taissia G Popova; Allison Teunis; Ruben Magni; Alessandra Luchini; Virginia Espina; Lance A Liotta; Serguei G Popov
Journal:  Nanomaterials (Basel)       Date:  2015-03       Impact factor: 5.076

5.  Core-shell hydrogel particles harvest, concentrate and preserve labile low abundance biomarkers.

Authors:  Caterina Longo; Alexis Patanarut; Tony George; Barney Bishop; Weidong Zhou; Claudia Fredolini; Mark M Ross; Virginia Espina; Giovanni Pellacani; Emanuel F Petricoin; Lance A Liotta; Alessandra Luchini
Journal:  PLoS One       Date:  2009-03-10       Impact factor: 3.240
  5 in total

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