M Stempelj1, I Ferjan. 1. Department of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia. mateja.stempelj@mf.uni-lj.si
Abstract
OBJECTIVE AND DESIGN: We investigated a signal transduction pathway involved in NGF induced histamine secretion from mast cells. We compared this mechanism with the exocytosis induced by basic secretagogue compound 48/80. MATERIALS AND METHODS: Isolated rat peritoneal mast cells were obtained from male Wistar rats. Histamine release was assayed spectrofluorometrically. RESULTS: We found that tyrosine kinase inhibitor genistein, phospholipase C (PLC) inhibitor U-73122, phosphatidylinositol 3-kinase (PI-3 kinase) inhibitor wortmannin, protein kinase C (PKC) inhibitors, staurosporine and GF109203X, but not MAP kinase inhibitors, SB203580 and PD98059, reduce histamine secretion in NGF provoked mast cell degranulation. In compound 48/80 mediated degranulation, we confirmed only the involvement of tyrosine kinase and PLC, but not PI-3 kinase, PKC and MAP kinases. CONCLUSIONS: Our results indicate that release of histamine from mast cells after stimulation with NGF is regulated by tyrosine kinase, PLC, PI-3 kinase and PKC, but not by MAP kinases. This biochemical pathway differs from that provoked by compound 48/80.
OBJECTIVE AND DESIGN: We investigated a signal transduction pathway involved in NGF induced histamine secretion from mast cells. We compared this mechanism with the exocytosis induced by basic secretagogue compound 48/80. MATERIALS AND METHODS: Isolated rat peritoneal mast cells were obtained from male Wistar rats. Histamine release was assayed spectrofluorometrically. RESULTS: We found that tyrosine kinase inhibitor genistein, phospholipase C (PLC) inhibitor U-73122, phosphatidylinositol 3-kinase (PI-3 kinase) inhibitor wortmannin, protein kinase C (PKC) inhibitors, staurosporine and GF109203X, but not MAP kinase inhibitors, SB203580 and PD98059, reduce histamine secretion in NGF provoked mast cell degranulation. In compound 48/80 mediated degranulation, we confirmed only the involvement of tyrosine kinase and PLC, but not PI-3 kinase, PKC and MAP kinases. CONCLUSIONS: Our results indicate that release of histamine from mast cells after stimulation with NGF is regulated by tyrosine kinase, PLC, PI-3 kinase and PKC, but not by MAP kinases. This biochemical pathway differs from that provoked by compound 48/80.
Authors: Jae Young Shin; Denis Nchang Che; Byoung Ok Cho; Hyun Ju Kang; Jisu Kim; Seon Il Jang Journal: Exp Ther Med Date: 2019-07-01 Impact factor: 2.447
Authors: Eva M J Peters; Sofia Liotiri; Eniko Bodó; Evelin Hagen; Tamás Bíró; Petra C Arck; Ralf Paus Journal: Am J Pathol Date: 2007-11-30 Impact factor: 4.307
Authors: Douglas P Jasmer; Bruce A Rosa; Rahul Tyagi; Christina A Bulman; Brenda Beerntsen; Joseph F Urban; Judy Sakanari; Makedonka Mitreva Journal: PLoS Negl Trop Dis Date: 2020-05-26