Literature DB >> 16158263

Purification and characterization of an extracellular cold-active serine protease from the psychrophilic bacterium Colwellia sp. NJ341.

Quan-Fu Wang1, Jin-Lai Miao, Yan-Hua Hou, Yu Ding, Guo-Dong Wang, Guang-You Li.   

Abstract

Colwellia sp. NJ341, isolated from Antarctic sea ice, secreted a cold-active serine protease. The purified protease had an apparent Mr of 60 kDa by SDS-PAGE and MALDI-TOF MS. It was active from pH 5-12 with maximum activity at 35 degrees C (assayed over 10 min). Activity at 0 degrees C was nearly 30% of the maximum activity. It was completely inhibited by phenylmethylsulfonyl fluoride.

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Year:  2005        PMID： 16158263     DOI： 10.1007/s10529-005-0016-x

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  11 in total

1.  Purification and Characterization of an Extracellular Low Temperature-Active and Alkaline Stable Peptidase from Psychrotrophic Acinetobacter sp. MN 12 MTCC (10786).

Authors:  Richa Salwan; Ramesh Chand Kasana
Journal:  Indian J Microbiol       Date:  2012-12-30       Impact factor: 2.461

2.  Purification and biochemical characterization of a cold-active lipase from Antarctic sea ice bacteria Pseudoalteromonas sp. NJ 70.

Authors:  Quanfu Wang; Yanhua Hou; Yu Ding; Peisheng Yan
Journal:  Mol Biol Rep       Date:  2012-06-20       Impact factor: 2.316

3.  Partial Characterization of α-Galactosidic Activity from the Antarctic Bacterial Isolate, Paenibacillus sp. LX-20 as a Potential Feed Enzyme Source.

Authors:  Inkyung Park; Jaekoo Lee; Jaiesoon Cho
Journal:  Asian-Australas J Anim Sci       Date:  2012-06       Impact factor: 2.509

4.  Crystal structure of a cold-active protease (Pro21717) from the psychrophilic bacterium, Pseudoalteromonas arctica PAMC 21717, at 1.4 Å resolution: Structural adaptations to cold and functional analysis of a laundry detergent enzyme.

Authors:  Ha Ju Park; Chang Woo Lee; Dockyu Kim; Hackwon Do; Se Jong Han; Jung Eun Kim; Bon-Hun Koo; Jun Hyuck Lee; Joung Han Yim
Journal:  PLoS One       Date:  2018-02-21       Impact factor: 3.240

5.  Characterization of a cold-active, detergent-stable metallopeptidase purified from Bacillus sp. S1DI 10 using Response Surface Methodology.

Authors:  Drishtant Singh; Sharad Thakur; Seema Madhumal Thayil; Anup Kumar Kesavan
Journal:  PLoS One       Date:  2019-05-23       Impact factor: 3.240

6.  Diversity and enzyme properties of protease-producing bacteria isolated from sub-Antarctic sediments of Isla de Los Estados, Argentina.

Authors:  Nelda L Olivera; Cynthia Sequeiros; Marina L Nievas
Journal:  Extremophiles       Date:  2007-02-15       Impact factor: 3.035

7.  Biotechnology of cold-active proteases.

Authors:  Swati Joshi; Tulasi Satyanarayana
Journal:  Biology (Basel)       Date:  2013-05-03

8.  Genomic and exoproteomic analyses of cold- and alkaline-adapted bacteria reveal an abundance of secreted subtilisin-like proteases.

Authors:  Jeanette E Lylloff; Lea B S Hansen; Morten Jepsen; Kristian W Sanggaard; Jan K Vester; Jan J Enghild; Søren J Sørensen; Peter Stougaard; Mikkel A Glaring
Journal:  Microb Biotechnol       Date:  2016-02-01       Impact factor: 5.813

9.  Draft Genome Sequences of Two Psychrotolerant Strains, Colwellia polaris MCCC 1C00015T and Colwellia chukchiensis CGMCC 1.9127T.

Authors:  Cong Zhang; Wenbin Guo; Yuguang Wang; Xinhua Chen
Journal:  Genome Announc       Date:  2018-01-25

Review 10.  Enzymes from Marine Polar Regions and Their Biotechnological Applications.

Authors:  Stefano Bruno; Daniela Coppola; Guido di Prisco; Daniela Giordano; Cinzia Verde
Journal:  Mar Drugs       Date:  2019-09-23       Impact factor: 5.118
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