Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae.

Literature DB >> 16157664

Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae.

Jewel A Daniel¹, Jiyoun Yoo, Blaine T Bettinger, David C Amberg, Daniel J Burke.

Abstract

Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3Delta1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5+ gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2005 PMID： 16157664 PMCID： PMC1456194 DOI： 10.1534/genetics.105.047662

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

4 in total

1. Global mapping of the yeast genetic interaction network.

Authors: Amy Hin Yan Tong; Guillaume Lesage; Gary D Bader; Huiming Ding; Hong Xu; Xiaofeng Xin; James Young; Gabriel F Berriz; Renee L Brost; Michael Chang; YiQun Chen; Xin Cheng; Gordon Chua; Helena Friesen; Debra S Goldberg; Jennifer Haynes; Christine Humphries; Grace He; Shamiza Hussein; Lizhu Ke; Nevan Krogan; Zhijian Li; Joshua N Levinson; Hong Lu; Patrice Ménard; Christella Munyana; Ainslie B Parsons; Owen Ryan; Raffi Tonikian; Tania Roberts; Anne-Marie Sdicu; Jesse Shapiro; Bilal Sheikh; Bernhard Suter; Sharyl L Wong; Lan V Zhang; Hongwei Zhu; Christopher G Burd; Sean Munro; Chris Sander; Jasper Rine; Jack Greenblatt; Matthias Peter; Anthony Bretscher; Graham Bell; Frederick P Roth; Grant W Brown; Brenda Andrews; Howard Bussey; Charles Boone
Journal: Science Date: 2004-02-06 Impact factor: 47.728

2. Systematic genetic analysis with ordered arrays of yeast deletion mutants.

Authors: A H Tong; M Evangelista; A B Parsons; H Xu; G D Bader; N Pagé; M Robinson; S Raghibizadeh; C W Hogue; H Bussey; B Andrews; M Tyers; C Boone
Journal: Science Date: 2001-12-14 Impact factor: 47.728

3. The a-factor pheromone of Saccharomyces cerevisiae is essential for mating.

Authors: S Michaelis; I Herskowitz
Journal: Mol Cell Biol Date: 1988-03 Impact factor: 4.272

4. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.

Authors: A Wach; A Brachat; R Pöhlmann; P Philippsen
Journal: Yeast Date: 1994-12 Impact factor: 3.239

4 in total

10 in total

1. Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.

Authors: Jewel A Daniel; Brice E Keyes; Yvonne P Y Ng; C Onyi Freeman; Daniel J Burke
Journal: Genetics Date: 2005-09-12 Impact factor: 4.562

2. Stringent mating-type-regulated auxotrophy increases the accuracy of systematic genetic interaction screens with Saccharomyces cerevisiae mutant arrays.

Authors: Indira Singh; Rebecca Pass; Sine Ozmen Togay; John W Rodgers; John L Hartman
Journal: Genetics Date: 2008-10-28 Impact factor: 4.562

3. Bioinformatic identification of genes suppressing genome instability.

Authors: Christopher D Putnam; Stephanie R Allen-Soltero; Sandra L Martinez; Jason E Chan; Tikvah K Hayes; Richard D Kolodner
Journal: Proc Natl Acad Sci U S A Date: 2012-11-05 Impact factor: 11.205

4. Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components.

Authors: John J Scarcelli; Susan Viggiano; Christine A Hodge; Catherine V Heath; David C Amberg; Charles N Cole
Journal: Genetics Date: 2008-08-09 Impact factor: 4.562

Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae.

1. Global mapping of the yeast genetic interaction network.

2. Systematic genetic analysis with ordered arrays of yeast deletion mutants.

3. The a-factor pheromone of Saccharomyces cerevisiae is essential for mating.

4. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.

1. Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.

2. Stringent mating-type-regulated auxotrophy increases the accuracy of systematic genetic interaction screens with Saccharomyces cerevisiae mutant arrays.

3. Bioinformatic identification of genes suppressing genome instability.

4. Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components.

5. Yeast based small molecule screen for inhibitors of SARS-CoV.

6. A strategy for extracting and analyzing large-scale quantitative epistatic interaction data.

7. Ubiquilin/Dsk2 promotes inclusion body formation and vacuole (lysosome)-mediated disposal of mutated huntingtin.

8. Auto-inhibition of Mif2/CENP-C ensures centromere-dependent kinetochore assembly in budding yeast.

Review 9. Yeast genetic interaction screens in the age of CRISPR/Cas.

10. Genetic interactions derived from high-throughput phenotyping of 6589 yeast cell cycle mutants.