Literature DB >> 16157554

Use of chromogenic medium in the isolation of yeasts from clinical specimens.

C K Murray1, M L Beckius1, J A Green1, D R Hospenthal1.   

Abstract

Over a 1 year period 3296 specimens submitted for fungal culture were plated onto routine mycological media (RM) and CHROMagar Candida (CaC) to evaluate the capability of CaC to improve on RM. With RM, cultures producing single yeast isolates were identified from 802 specimens. CaC produced similar results, with 76 % agreement. Of 761 specimens that yielded a single Candida species by RM, 615 (81 %) produced one or more yeast isolates using CaC. Of concern, 132 negative CaC cultures corresponded to specimens that yielded C. albicans alone on RM. When yeasts were recovered, CaC correctly identified 98 % of C. albicans, 93 % of Candida tropicalis, 96 % of Candida glabrata and 100 % of Candida krusei based on typical colours. CaC did potentially improve on RM by detecting yeasts in 91 specimens that yielded none by routine methods. CaC was noted to recover more yeast isolates than RM when mixed cultures were detected. Overall, the role of CaC in improving RM appears limited.

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Year:  2005        PMID: 16157554     DOI: 10.1099/jmm.0.45942-0

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN: 0022-2615            Impact factor:   2.472


  14 in total

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6.  Presumptive identification of Candida species other than C. albicans, C. krusei, and C. tropicalis with the chromogenic medium CHROMagar Candida.

Authors:  Duane R Hospenthal; Miriam L Beckius; Karon L Floyd; Lynn L Horvath; Clinton K Murray
Journal:  Ann Clin Microbiol Antimicrob       Date:  2006-01-03       Impact factor: 3.944

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9.  Evaluation of chromogenic media and seminested PCR in the identification of Candida species.

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Journal:  Braz J Microbiol       Date:  2014-05-02       Impact factor: 2.476

10.  Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures.

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Journal:  PLoS One       Date:  2020-03-23       Impact factor: 3.240

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