| Literature DB >> 16157260 |
David M Whiley1, Theo P Sloots.
Abstract
This study was designed to assess the effect of sequence mismatches between primers and their targets on viral quantitative PCR. Numerous primers were constructed incorporating various mismatches with a target sequence on the BKV T antigen gene. When using these primers in standard Taqman two-step cycling conditions, as few as two mismatches in a single primer increased cycle threshold values and significantly influenced the calculation of viral load.Mesh：
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Year: 2005 PMID： 16157260 DOI： 10.1016/j.jcv.2005.02.010
Source DB: PubMed Journal: J Clin Virol ISSN： 1386-6532 Impact factor: 3.168