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Literature DB >> 16157212

An improved arbitrary primed PCR method for rapid characterization of transposon insertion sites.

Sankar Das¹, Jody C Noe, Sehmi Paik, Todd Kitten.

Abstract

Modifications were made to published arbitrary primed polymerase chain reaction (AP-PCR) procedures that resulted in increased specificity and sensitivity. Several arbitrary primer sequences were also evaluated, resulting in recommendations for primer design.

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Year: 2005 PMID： 16157212 DOI： 10.1016/j.mimet.2005.02.011

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

47 in total

1. Forward genetic in planta screen for identification of plant-protective traits of Sphingomonas sp. strain Fr1 against Pseudomonas syringae DC3000.

Authors: Christine Vogel; Gerd Innerebner; Judith Zingg; Jan Guder; Julia A Vorholt
Journal: Appl Environ Microbiol Date: 2012-06-01 Impact factor: 4.792

2. Constitutive expression of the maltoporin LamB in the absence of OmpR damages the cell envelope.

Authors: Sylvia A Reimann; Alan J Wolfe
Journal: J Bacteriol Date: 2010-12-03 Impact factor: 3.490

3. Identification of virulence determinants for endocarditis in Streptococcus sanguinis by signature-tagged mutagenesis.

Authors: Sehmi Paik; Lauren Senty; Sankar Das; Jody C Noe; Cindy L Munro; Todd Kitten
Journal: Infect Immun Date: 2005-09 Impact factor: 3.441

4. Functional Characterization of a 28-Kilobase Catabolic Island from Pseudomonas sp. Strain M1 Involved in Biotransformation of β-Myrcene and Related Plant-Derived Volatiles.

Authors: Pedro Soares-Castro; Pedro Montenegro-Silva; Hermann J Heipieper; Pedro M Santos
Journal: Appl Environ Microbiol Date: 2017-04-17 Impact factor: 4.792

5. The two-component regulators GacS and GacA positively regulate a nonfluorescent siderophore through the Gac/Rsm signaling cascade in high-siderophore-yielding Pseudomonas sp. strain HYS.

Authors: Xinyan Yu; Min Chen; Zhen Jiang; Yi Hu; Zhixiong Xie
Journal: J Bacteriol Date: 2014-06-30 Impact factor: 3.490

An improved arbitrary primed PCR method for rapid characterization of transposon insertion sites.

1. Forward genetic in planta screen for identification of plant-protective traits of Sphingomonas sp. strain Fr1 against Pseudomonas syringae DC3000.

2. Constitutive expression of the maltoporin LamB in the absence of OmpR damages the cell envelope.

3. Identification of virulence determinants for endocarditis in Streptococcus sanguinis by signature-tagged mutagenesis.

4. Functional Characterization of a 28-Kilobase Catabolic Island from Pseudomonas sp. Strain M1 Involved in Biotransformation of β-Myrcene and Related Plant-Derived Volatiles.

5. The two-component regulators GacS and GacA positively regulate a nonfluorescent siderophore through the Gac/Rsm signaling cascade in high-siderophore-yielding Pseudomonas sp. strain HYS.

6. PBP1a/LpoA but not PBP1b/LpoB are involved in regulation of the major β-lactamase gene blaA in Shewanella oneidensis.

7. Many chromosomal genes modulate MarA-mediated multidrug resistance in Escherichia coli.

8. A molybdopterin oxidoreductase is involved in H2 oxidation in Desulfovibrio desulfuricans G20.

9. A PhoPQ-Regulated ABC Transporter System Exports Tetracycline in Pseudomonas aeruginosa.

10. Exoprotein production correlates with morphotype changes of nonmotile Shewanella oneidensis mutants.