Literature DB >> 16155807

Development of an antigen ELISA to detect sapovirus in clinical stool specimens.

G S Hansman1, R Guntapong, Y Pongsuwanna, K Natori, K Katayama, N Takeda.   

Abstract

Human sapovirus (SaV) strains are etiological agents of mild and/or acute gastroenteritis in children and adults. In this study, we describe the development of a novel antigen enzyme-linked immunosorbent assay (ELISA) detection system that was based on hyperimmune rabbit and guinea pig antisera raised against SaV genogroup I (GI) virus-like particles. The ELISA had 100% specificity, and sensitivities of 60% and 25% when compared to single-round PCR and nested PCR, respectively. Our results have shown the ELISA was useful in detecting SaV GI antigens in clinical stool specimens collected two days after the onset of illness.

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Year:  2005        PMID： 16155807     DOI： 10.1007/s00705-005-0630-x

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  6 in total

1.  Outbreak of gastroenteritis due to sapovirus.

Authors:  Grant S Hansman; Hiroyuki Saito; Chihiro Shibata; Shizuko Ishizuka; Mitsuaki Oseto; Tomoichiro Oka; Naokazu Takeda
Journal:  J Clin Microbiol       Date:  2007-01-31       Impact factor: 5.948

Review 2.  Comprehensive review of human sapoviruses.

Authors:  Tomoichiro Oka; Qiuhong Wang; Kazuhiko Katayama; Linda J Saif
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

3.  Nanobody binding to a conserved epitope promotes norovirus particle disassembly.

Authors:  Anna D Koromyslova; Grant S Hansman
Journal:  J Virol       Date:  2014-12-17       Impact factor: 5.103

4.  Antigenic diversity of human sapoviruses.

Authors:  Grant S Hansman; Tomoichiro Oka; Naomi Sakon; Naokazu Takeda
Journal:  Emerg Infect Dis       Date:  2007-10       Impact factor: 6.883

5.  RECOVIR: an application package to automatically identify some single stranded RNA viruses using capsid protein residues that uniquely distinguish among these viruses.

Authors:  Dianhui Zhu; George E Fox; Sugoto Chakravarty
Journal:  BMC Bioinformatics       Date:  2007-10-10       Impact factor: 3.169

6.  Prevalence of sapovirus infection among infant and adult patients with acute gastroenteritis in Tehran, Iran.

Authors:  Sara Romani; Pedram Azimzadeh; Seyed Reza Mohebbi; Sajad Majidizadeh Bozorgi; Narges Zali; Farzaneh Jadali
Journal:  Gastroenterol Hepatol Bed Bench       Date:  2012
  6 in total

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