A new model system for investigation of ionic channels in filamentous fungi: evidence for existence of two K+-permeable ionic channels in Phycomyces blakesleeanus.

A technique was developed to obtain viable cytoplasmic droplets, enabling the formation of a gigaohm seal. Such cytoplasmic droplets can be used for characterization of plasma membrane ion channels from filamentous fungi by patch-clamp technique. Two K(+) ionic channels are characterized with a conductance of 43 pS and 74 pS.