Literature DB >> 16153258

PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosome-borne orfX.

C Cuny1, W Witte.   

Abstract

The chromosomal location of the SCCmec elements containing mecA allows the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains by PCR amplification of a sequence covering the right junction of the SCCmec elements and the adjacent chromosomal region encoding the species-specific ORFX. MRSA strains can be identified specifically using one forward primer, with only one or two mismatches, targeting the SCCmec elements of different types, and one reverse primer targeting the orfX region.

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Year:  2005        PMID： 16153258     DOI： 10.1111/j.1469-0691.2005.01236.x

Source DB:  PubMed          Journal:  Clin Microbiol Infect        ISSN： 1198-743X            Impact factor:   8.067


  25 in total

1.  Detection of methicillin-resistant Staphylococcus aureus (MRSA) from nasal samples by multiplex real-time PCR based on dual priming AT-rich primers.

Authors:  M K Yadav; S K Kwon; H J Huh; S-W Chae; J-J Song
Journal:  Folia Microbiol (Praha)       Date:  2011-12-22       Impact factor: 2.099

2.  Does nasal cocolonization by methicillin-resistant coagulase-negative staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods?

Authors:  Karsten Becker; Isabelle Pagnier; Brigitte Schuhen; Frauke Wenzelburger; Alexander W Friedrich; Frank Kipp; Georg Peters; Christof von Eiff
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

3.  Be aware of the possibility of false-positive results in single-locus PCR assays for methicillin-resistant Staphylococcus aureus.

Authors:  Jan Rupp; Ines Fenner; Werner Solbach; Jens Gieffers
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

4.  Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus.

Authors:  Patrice Francois; Manuela Bento; Gesuele Renzi; Stephan Harbarth; Didier Pittet; Jacques Schrenzel
Journal:  J Clin Microbiol       Date:  2007-04-11       Impact factor: 5.948

5.  Bacterial contamination of unused, disposable non-sterile gloves on a hospital orthopaedic ward.

Authors:  Kim A Hughes; Jon Cornwall; Jean-Claude Theis; Heather J L Brooks
Journal:  Australas Med J       Date:  2013-06-30

6.  A general method for rapid determination of antibiotic susceptibility and species in bacterial infections.

Authors:  Anja Mezger; Erik Gullberg; Jenny Göransson; Anna Zorzet; David Herthnek; Eva Tano; Mats Nilsson; Dan I Andersson
Journal:  J Clin Microbiol       Date:  2014-11-19       Impact factor: 5.948

7.  Accurate Detection of Methicillin-Resistant Staphylococcus aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR.

Authors:  Jun Luo; Junhua Li; Hang Yang; Junping Yu; Hongping Wei
Journal:  J Clin Microbiol       Date:  2017-07-19       Impact factor: 5.948

8.  Detection of staphylococcal cassette chromosome mec-associated DNA segments in multiresistant methicillin-susceptible Staphylococcus aureus (MSSA) and identification of Staphylococcus epidermidis ccrAB4 in both methicillin-resistant S. aureus and MSSA.

Authors:  Anna C Shore; Angela S Rossney; Brian O'Connell; Celine M Herra; Derek J Sullivan; Hilary Humphreys; David C Coleman
Journal:  Antimicrob Agents Chemother       Date:  2008-10-13       Impact factor: 5.191

9.  Evaluation of single and double-locus real-time PCR assays for methicillin-resistant Staphylococcus aureus (MRSA) surveillance.

Authors:  Sara Kolman; Haya Arielly; Yossi Paitan
Journal:  BMC Res Notes       Date:  2010-04-21

Review 10.  Rapid detection of methicillin-resistant Staphylococcus aureus directly from clinical samples: methods, effectiveness and cost considerations.

Authors:  Enno Stürenburg
Journal:  Ger Med Sci       Date:  2009-07-06
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