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Literature DB >> 16151164

Rapid and quantitative detection of hepatitis A virus from green onion and strawberry rinses by use of real-time reverse transcription-PCR.

Abstract

In this study, an immunomagnetic capture method and a real-time reverse transcription-PCR assay were used to quantify hepatitis A virus (HAV) in green onion and strawberry rinses. This combined protocol detected as low as 0.5 PFU HAV in produce rinses and concentrated HAV levels up to 20-fold.

Entities: Species

Mesh：

Substances：
Buffers
RNA, Viral

Year: 2005 PMID： 16151164 PMCID： PMC1214659 DOI： 10.1128/AEM.71.9.5624-5626.2005

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

19 in total

Review 1. Pre-PCR processing: strategies to generate PCR-compatible samples.

Authors: Peter Rådström; Rickard Knutsson; Petra Wolffs; Maria Lövenklev; Charlotta Löfström
Journal: Mol Biotechnol Date: 2004-02 Impact factor: 2.695

2. Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus.

Authors: T Cromeans; M D Sobsey; H A Fields
Journal: J Med Virol Date: 1987-05 Impact factor: 2.327

3. Monoclonal antibodies against hepatitis A virus.

Authors: A MacGregor; M Kornitschuk; J G Hurrell; N I Lehmann; A G Coulepis; S A Locarnini; I D Gust
Journal: J Clin Microbiol Date: 1983-11 Impact factor: 5.948

4. An outbreak of hepatitis A associated with green onions.

Authors: C M Dentinger; W A Bower; O V Nainan; S M Cotter; G Myers; L M Dubusky; S Fowler; E D Salehi; B P Bell
Journal: J Infect Dis Date: 2001-03-26 Impact factor: 5.226

5. Rapid concentration and detection of hepatitis A virus from lettuce and strawberries.

Authors: S Bidawid; J M Farber; S A Sattar
Journal: J Virol Methods Date: 2000-08 Impact factor: 2.014

6. Improved method for the recovery of hepatitis A virus from oysters.

Authors: J L Mullendore; M D Sobsey; Y C Shieh
Journal: J Virol Methods Date: 2001-05 Impact factor: 2.014

7. Quantification and duration of viraemia during hepatitis A infection as determined by real-time RT-PCR.

Authors: M Costa-Mattioli; S Monpoeho; E Nicand; M-H Aleman; S Billaudel; V Ferré
Journal: J Viral Hepat Date: 2002-03 Impact factor: 3.728

8. Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples.

Authors: Khaled H Abd El Galil; M A El Sokkary; S M Kheira; Andre M Salazar; Marylynn V Yates; Wilfred Chen; Ashok Mulchandani
Journal: Appl Environ Microbiol Date: 2004-07 Impact factor: 4.792

Rapid and quantitative detection of hepatitis A virus from green onion and strawberry rinses by use of real-time reverse transcription-PCR.

Review 1. Pre-PCR processing: strategies to generate PCR-compatible samples.

2. Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus.

3. Monoclonal antibodies against hepatitis A virus.

4. An outbreak of hepatitis A associated with green onions.

5. Rapid concentration and detection of hepatitis A virus from lettuce and strawberries.

6. Improved method for the recovery of hepatitis A virus from oysters.

7. Quantification and duration of viraemia during hepatitis A infection as determined by real-time RT-PCR.

8. Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples.

9. Viral and clinical factors associated with the fulminant course of hepatitis A infection.

Review 10. Food-related illness and death in the United States.

1. A rapid single-tube protocol for HAV detection by nested real-time PCR.

2. Procedure for rapid concentration and detection of enteric viruses from berries and vegetables.

3. Development of lectin-linked immunomagnetic separation for the detection of hepatitis a virus.