| Literature DB >> 16150445 |
J A Encinar1, M L Molina, J A Poveda, F N Barrera, M L Renart, A M Fernández, J M González-Ros.
Abstract
The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440-446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1-125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions.Mesh：
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Year: 2005 PMID： 16150445 DOI： 10.1016/j.febslet.2005.08.038
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124