BACKGROUND: The measurement of morphine in biological samples has become a routine in many clinical and forensic toxicology laboratories. A high-performance liquid chromatography (HPLC) method was developed for the determination of morphine in plasma. METHODS: Samples were extracted using Zeolite Y column followed by reversed phase HPLC with fluorescence detection. This method was based on an ex-calibration procedure and was linear between 20 and 200 ng/ml of morphine. Blood from 10 male opiate addicts were obtained from Rosbeh Hospital. All of the male smoked opiate (heroin, opium) and cigarettes. RESULTS: The mean total level 5 h after the last abuse was 152.4 ng/ml and 37.6 ng/ml at 10-15 h. The method was reliable for morphine determination in blood even after 5 half-lives after the last abuse. CONCLUSIONS: This method is simple and rapid and may be useful for routine monitoring of plasma morphine concentration.
BACKGROUND: The measurement of morphine in biological samples has become a routine in many clinical and forensic toxicology laboratories. A high-performance liquid chromatography (HPLC) method was developed for the determination of morphine in plasma. METHODS: Samples were extracted using Zeolite Y column followed by reversed phase HPLC with fluorescence detection. This method was based on an ex-calibration procedure and was linear between 20 and 200 ng/ml of morphine. Blood from 10 male opiate addicts were obtained from Rosbeh Hospital. All of the male smoked opiate (heroin, opium) and cigarettes. RESULTS: The mean total level 5 h after the last abuse was 152.4 ng/ml and 37.6 ng/ml at 10-15 h. The method was reliable for morphine determination in blood even after 5 half-lives after the last abuse. CONCLUSIONS: This method is simple and rapid and may be useful for routine monitoring of plasma morphine concentration.
Authors: Crystal R Leibrand; Jason J Paris; Austin M Jones; Quamrun N Masuda; Matthew S Halquist; Woong-Ki Kim; Pamela E Knapp; Angela D M Kashuba; Kurt F Hauser; MaryPeace McRae Journal: J Neurovirol Date: 2019-05-17 Impact factor: 2.643
Authors: Sylvia Fitting; David L Stevens; Fayez A Khan; Krista L Scoggins; Rachel M Enga; Patrick M Beardsley; Pamela E Knapp; William L Dewey; Kurt F Hauser Journal: J Pharmacol Exp Ther Date: 2015-11-05 Impact factor: 4.030
Authors: William D Marks; Jason J Paris; Aaron J Barbour; Jean Moon; Valerie J Carpenter; Virginia D McLane; Arianna R S Lark; Sara R Nass; Jingli Zhang; Viktor Yarotskyy; A Rory McQuiston; Pamela E Knapp; Kurt F Hauser Journal: eNeuro Date: 2021-05-24