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Literature DB >> 1614857

Structural and functional features of cis-acting sequences in the basic replicon of plasmid ColIb-P9.

K Tanaka¹, H Sakai, Y Honda, T Nakamura, A Higashi, T Komano.

Abstract

We have structurally and functionally analyzed the cis-elements essential for ColIb-P9 plasmid DNA replication. The putative oriV region encompassed a region of 172 base pairs (bp) located 152 bp downstream of the repZ gene. A typical dnaA box found in this region proved nonessential for the DNA replication of ColIb-P9. The ssi signal of ColIb-P9 is a homologue of the G-sites of R1 and R100 plasmids. Deletion of the G-site led to 1.5-fold reduction of the copy number, suggesting that although this G-site is not essential, it is important for efficient ColIb-P9 DNA replication. In addition, the ColIb-P9 replicon is highly and extensively homologous with the P307 (RepFIC) replicon, and highly homologous with the R100 (RepFIIA) replicon around the G-site region. These facts imply a common ancestry from which the plasmids have evolved.

Entities: Species

Mesh：

Substances：
DNA, Bacterial

Year: 1992 PMID： 1614857 PMCID： PMC336911 DOI： 10.1093/nar/20.11.2705

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

30 in total

1. DnaA protein/DNA interaction. Modulation of the recognition sequence.

Authors: C Schaefer; W Messer
Journal: Mol Gen Genet Date: 1991-04

2. Leading strand synthesis of R1 plasmid replication in vitro is primed by primase alone at a specific site downstream of oriR.

Authors: H Masai; K Arai
Journal: J Biol Chem Date: 1989-05-15 Impact factor: 5.157

3. RepA and DnaA proteins are required for initiation of R1 plasmid replication in vitro and interact with the oriR sequence.

Authors: H Masai; K Arai
Journal: Proc Natl Acad Sci U S A Date: 1987-07 Impact factor: 11.205

Review 4. A model for initiation at origins of DNA replication.

Authors: D Bramhill; A Kornberg
Journal: Cell Date: 1988-09-23 Impact factor: 41.582

5. The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.

Authors: J W Taylor; W Schmidt; R Cosstick; A Okruszek; F Eckstein
Journal: Nucleic Acids Res Date: 1985-12-20 Impact factor: 16.971

6. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.

Authors: J W Taylor; J Ott; F Eckstein
Journal: Nucleic Acids Res Date: 1985-12-20 Impact factor: 16.971

7. Distribution of basic replicons having homology with RepFIA, RepFIB, and RepFIC among IncF group plasmids.

Authors: P L Bergquist; S Saadi; W K Maas
Journal: Plasmid Date: 1986-01 Impact factor: 3.466

8. Nucleotide sequence analysis of RepFIC, a basic replicon present in IncFI plasmids P307 and F, and its relation to the RepA replicon of IncFII plasmids.

Authors: S Saadi; W K Maas; D F Hill; P L Bergquist
Journal: J Bacteriol Date: 1987-05 Impact factor: 3.490

9. Functional division and reconstruction of a plasmid replication origin: molecular dissection of the oriV of the broad-host-range plasmid RSF1010.

Authors: Y Honda; H Sakai; H Hiasa; K Tanaka; T Komano; M Bagdasarian
Journal: Proc Natl Acad Sci U S A Date: 1991-01-01 Impact factor: 11.205

10. Requirement of the Escherichia coli dnaA gene function for integrative suppression of dnaA mutations by plasmid R 100-1.

Authors: T Nagata; Y Murakami; M Imai
Journal: Mol Gen Genet Date: 1988-07

6 in total

1. Copy number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA binding at a specific RNA site.

Authors: K Asano; K Mizobuchi
Journal: EMBO J Date: 1998-09-01 Impact factor: 11.598

6. Comparative analysis of functional and structural features in the primase-dependent priming signals, G sites, from phages and plasmids.

Authors: K Tanaka; T Rogi; H Hiasa; D M Miao; Y Honda; N Nomura; H Sakai; T Komano
Journal: J Bacteriol Date: 1994-06 Impact factor: 3.490